AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI
The issue of resistance in malarial infection makes development of novel drugs a necessity. An alternative source for discovering such drugs is natural products. Andrographis paniculata Nees which known as sambiloto was traditionally used for antimalaria in Indonesia. Therefore, the aim for this...
Saved in:
Main Author: | |
---|---|
Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2016
|
Subjects: | |
Online Access: | http://repository.unair.ac.id/65088/1/FF.FT.03.17%20.%20Ast.a%20-%20ABSTRAK.pdf http://repository.unair.ac.id/65088/2/FF.FT.03.17%20.%20Ast.a%20-%20SEC.pdf http://repository.unair.ac.id/65088/ http://lib.unair.ac.id |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
id |
id-langga.65088 |
---|---|
record_format |
dspace |
spelling |
id-langga.650882018-01-17T19:46:58Z http://repository.unair.ac.id/65088/ AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI DERY ASTRIANTO, 051211132085 RS1-441 Pharmacy and materia medica The issue of resistance in malarial infection makes development of novel drugs a necessity. An alternative source for discovering such drugs is natural products. Andrographis paniculata Nees which known as sambiloto was traditionally used for antimalaria in Indonesia. Therefore, the aim for this study was to develop A. paniculata as a new phytopharmaceutical drug. The study was began for determining antimalaria acitivity of raw material product. The material are Etil Asetat fraction that obtained from ethanol extract 96% and ethanol extract 70% of A. paniculata herbs. Then comparing between the two materials that give better activity determined by the value of each ED50 as reference to be manufactured as phytopharmaceutical drug. In vivo antimalarial assay on Plasmodium berghei infected mice was carried out by oral administration, twice a day for four consecutive days based on Peter’s 4 days suppressive test. Each EA fraction was given at a dose which equal to 6.25; 12.5; 25; and 50 mg andrographolide per kg mice body weight. The observation was done for a week (H0-H6) and everyday thin films were made from the tail blood of each mouse. The parasitemia level was determined by counting the number of parasitized erythrocytes out of 1000 normal erythrocytes in random fields of the microscope. Antimalarial test results showed that antimalarial activity of EA fraction-96 higher than EA fraction-70 with ED50 4.216 mg/kg BW and 6.747 mg/kg BW respectively. 2016 Thesis NonPeerReviewed text id http://repository.unair.ac.id/65088/1/FF.FT.03.17%20.%20Ast.a%20-%20ABSTRAK.pdf text id http://repository.unair.ac.id/65088/2/FF.FT.03.17%20.%20Ast.a%20-%20SEC.pdf DERY ASTRIANTO, 051211132085 (2016) AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI. Skripsi thesis, Universitas Airlangga. http://lib.unair.ac.id |
institution |
Universitas Airlangga |
building |
Universitas Airlangga Library |
country |
Indonesia |
collection |
UNAIR Repository |
language |
Indonesian Indonesian |
topic |
RS1-441 Pharmacy and materia medica |
spellingShingle |
RS1-441 Pharmacy and materia medica DERY ASTRIANTO, 051211132085 AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI |
description |
The issue of resistance in malarial infection makes
development of novel drugs a necessity. An alternative source for
discovering such drugs is natural products. Andrographis paniculata
Nees which known as sambiloto was traditionally used for
antimalaria in Indonesia. Therefore, the aim for this study was to
develop A. paniculata as a new phytopharmaceutical drug. The study
was began for determining antimalaria acitivity of raw material
product. The material are Etil Asetat fraction that obtained from
ethanol extract 96% and ethanol extract 70% of A. paniculata herbs.
Then comparing between the two materials that give better activity
determined by the value of each ED50 as reference to be
manufactured as phytopharmaceutical drug. In vivo antimalarial
assay on Plasmodium berghei infected mice was carried out by oral
administration, twice a day for four consecutive days based on
Peter’s 4 days suppressive test. Each EA fraction was given at a dose
which equal to 6.25; 12.5; 25; and 50 mg andrographolide per kg
mice body weight. The observation was done for a week (H0-H6) and
everyday thin films were made from the tail blood of each mouse. The parasitemia level was determined by counting the number of
parasitized erythrocytes out of 1000 normal erythrocytes in random
fields of the microscope. Antimalarial test results showed that
antimalarial activity of EA fraction-96 higher than EA fraction-70
with ED50 4.216 mg/kg BW and 6.747 mg/kg BW respectively. |
format |
Theses and Dissertations NonPeerReviewed |
author |
DERY ASTRIANTO, 051211132085 |
author_facet |
DERY ASTRIANTO, 051211132085 |
author_sort |
DERY ASTRIANTO, 051211132085 |
title |
AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI |
title_short |
AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI |
title_full |
AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI |
title_fullStr |
AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI |
title_full_unstemmed |
AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI |
title_sort |
aktivitas antimalaria fraksi etil asetat-96 dan fraksi etil asetat-70 herba sambiloto (andrographis paniculata nees) secara in vivo pada mencit yang diinfeksi plasmodium berghei |
publishDate |
2016 |
url |
http://repository.unair.ac.id/65088/1/FF.FT.03.17%20.%20Ast.a%20-%20ABSTRAK.pdf http://repository.unair.ac.id/65088/2/FF.FT.03.17%20.%20Ast.a%20-%20SEC.pdf http://repository.unair.ac.id/65088/ http://lib.unair.ac.id |
_version_ |
1681148869395611648 |