PATOGENESIS Trypanosoma evansi ISOLAT SUMBA (372) PADA TIKUS PUTIH (Rattus novergicus) DAN IDENTIFIKASI MENGGUNAKAN PRIMER BERBEDA DENGAN METODE Polymerase Chain Reaction
Surra is a zoonotic disease caused by the protozoan Trypanosoma evansi. In Indonesia, the disease is one of the endemic diseases and attacking a lot of livestocks and wild animals. One region of endemic surra in Indonesia is Sumba. The purpose of this research was to study the pathogenesis of Try...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/66722/1/TPKMV.10.17%20.%20Kum.p%20-%20ABSTRAK.pdf http://repository.unair.ac.id/66722/2/TPKMV.10.17%20.%20Kum.p%20-%20SEC.pdf http://repository.unair.ac.id/66722/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Surra is a zoonotic disease caused by the protozoan Trypanosoma evansi.
In Indonesia, the disease is one of the endemic diseases and attacking a lot of
livestocks and wild animals. One region of endemic surra in Indonesia is Sumba.
The purpose of this research was to study the pathogenesis of Trypanosoma
evansi isolate from Sumba and to identification of Trypanosoma evansi using
differences primers with Polymerase Chain Reaction (PCR) methode. This study
used 12 rats (Rattus norvegicus) strain Sprague dawley was divided into two
replications. P1: Trypanosoma evansi was infected in rats (Rattus norvegicus)
with 1x101 of dose infections and P2: Trypanosoma evansi was infected in rats
(Rattus norvegicus) with 1x104 of dose infections. Trypanosoma evansi isolate
Sumba with the different infection dose can be decrease the blood glucose and
PCV. Rats with high dose infection (P2), the decrease until to the extent of normal
value. Trypanosoma evansi isolate Sumba was detected on the first day of the
infection in both doses using primer ITS1 and TBR1/2. |
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