PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica) TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK
Introduction: Cigarette smoke exposure is a source of free radicals that can lead to oxidative stress. A plant variety which potentially act as an antioxidant is broccoli (Brassica oleracea var. italica). Broccoli is a rich source of ascorbic acid, carotene and phenols. One of possible product of...
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id-langga.684912017-12-20T18:32:12Z http://repository.unair.ac.id/68491/ PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica) TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK Rizka Fitriani, NIM011411131084 R Medicine Introduction: Cigarette smoke exposure is a source of free radicals that can lead to oxidative stress. A plant variety which potentially act as an antioxidant is broccoli (Brassica oleracea var. italica). Broccoli is a rich source of ascorbic acid, carotene and phenols. One of possible product of broccoli is broccoli juice. The objectives of this research was to evaluate the antioxidant activity of broccoli juice, using serum Malondialdehyde (MDA) level assay. Method: This research is a true experimental laboratory research with posttest only control group design. 27 samples used were male Wistar rats and were divided into three groups which consisted of one negative control group (n = 9), one positive control group (n = 9), and one treatment group (n = 9). Animals in the negative control group are not exposed to cigarette smoke. The positive control group exposed to cigarette smoke as much as four cigarettes per day spent for one hour. The treatment group given broccoli juice at a dose of 2.5 g/kgBW and exposed to cigarette smoke as much as four cigarettes a day spent for one hour. All groups receive the treatment for 14 days. Result: Median serum MDA level in the positive control group was 7.60 (range, 5.19-8.98), in the negative control group 4.62 (range, 3.47-7.14), and in the treatment group 3.24 (range, 2.56-11.61). After data analysis, it is known that there are significant differences from the three groups (p = 0.029). It was found that there was a significant difference between the positive control group and the negative control group (p=0.007), there was no significant difference between the positive control group and the treatment group (p = 0.070), and there was no significant difference between negative control group and treatment group (p = 0.289). Conclusion: Despite a trend in lowering serum MDA levels, broccoli juice in this study showed insignificant effect against oxidative stress caused by cigarette smoke exposure in the laboratory animals. 2017-12-21 Thesis NonPeerReviewed text en http://repository.unair.ac.id/68491/1/abstrak.pdf text en http://repository.unair.ac.id/68491/2/full%20text.pdf Rizka Fitriani, NIM011411131084 (2017) PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica) TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK. Skripsi thesis, Universitas Airlangga. |
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R Medicine Rizka Fitriani, NIM011411131084 PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica) TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK |
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Introduction: Cigarette smoke exposure is a source of free radicals that can
lead to oxidative stress. A plant variety which potentially act as an antioxidant is
broccoli (Brassica oleracea var. italica). Broccoli is a rich source of ascorbic acid,
carotene and phenols. One of possible product of broccoli is broccoli juice. The
objectives of this research was to evaluate the antioxidant activity of broccoli juice,
using serum Malondialdehyde (MDA) level assay.
Method: This research is a true experimental laboratory research with
posttest only control group design. 27 samples used were male Wistar rats and were
divided into three groups which consisted of one negative control group (n = 9), one
positive control group (n = 9), and one treatment group (n = 9). Animals in the
negative control group are not exposed to cigarette smoke. The positive control
group exposed to cigarette smoke as much as four cigarettes per day spent for one
hour. The treatment group given broccoli juice at a dose of 2.5 g/kgBW and
exposed to cigarette smoke as much as four cigarettes a day spent for one hour. All
groups receive the treatment for 14 days.
Result: Median serum MDA level in the positive control group was 7.60
(range, 5.19-8.98), in the negative control group 4.62 (range, 3.47-7.14), and in the
treatment group 3.24 (range, 2.56-11.61). After data analysis, it is known that there
are significant differences from the three groups (p = 0.029). It was found that there
was a significant difference between the positive control group and the negative
control group (p=0.007), there was no significant difference between the positive
control group and the treatment group (p = 0.070), and there was no significant
difference between negative control group and treatment group (p = 0.289).
Conclusion: Despite a trend in lowering serum MDA levels, broccoli juice
in this study showed insignificant effect against oxidative stress caused by cigarette
smoke exposure in the laboratory animals. |
format |
Theses and Dissertations NonPeerReviewed |
author |
Rizka Fitriani, NIM011411131084 |
author_facet |
Rizka Fitriani, NIM011411131084 |
author_sort |
Rizka Fitriani, NIM011411131084 |
title |
PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica)
TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN
GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK |
title_short |
PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica)
TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN
GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK |
title_full |
PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica)
TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN
GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK |
title_fullStr |
PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica)
TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN
GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK |
title_full_unstemmed |
PENGARUH PEMBERIAN JUS BROKOLI (Brassica oleracea var. italica)
TERHADAP KADAR MALONDIALDEHID (MDA) Rattus norvegicus JANTAN
GALUR WISTAR AKIBAT PAPARAN ASAP ROKOK |
title_sort |
pengaruh pemberian jus brokoli (brassica oleracea var. italica)
terhadap kadar malondialdehid (mda) rattus norvegicus jantan
galur wistar akibat paparan asap rokok |
publishDate |
2017 |
url |
http://repository.unair.ac.id/68491/1/abstrak.pdf http://repository.unair.ac.id/68491/2/full%20text.pdf http://repository.unair.ac.id/68491/ |
_version_ |
1681149435846852608 |