Effect of Caffeine on Morphology of Epididymis Spermatozoa of Bali Bull
There is an unexpected loss of breeding animals of high genetic value for any reason, such as loss of libido, reproductive tract injury, or death. This loss can be reduced by harvesting spermatozoa from the epididymis with cryopreservation. However, after cryopreservation,...
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| Main Authors: | , , , |
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| Format: | Book PeerReviewed |
| Language: | English English Indonesian |
| Published: |
IPB Bogor
2015
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/74082/2/Bukti%208%20Effect%20of%20Caffeine%20on%20Morphology%20of%20.......pdf http://repository.unair.ac.id/74082/1/Bukti%208%20Effect%20of%20Caffeine%20on%20Morphology%20of%20Epididymis%20Spermatozoa%20of%20Bali%20Bull%20%282%29.pdf http://repository.unair.ac.id/74082/3/Penilaian%208%20Effect%20of%20Caffeine%20on%20Morphology%20of%20.......pdf http://repository.unair.ac.id/74082/ http://fapet.ipb.ac.id/~isai/pdf/ISAI_%202015_3_2015.pdf |
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| Institution: | Universitas Airlangga |
| Language: | English English Indonesian |
| Summary: | There is an unexpected loss of breeding animals of high genetic value for any reason, such as loss of
libido, reproductive tract injury, or death. This loss can be reduced by harvesting spermatozoa from the
epididymis with cryopreservation. However, after cryopreservation, there is still a decline of 50% in sperm
post-thaw viability after thawing process. This research was conducted to evaluate the use of caffeine to
improve cryopreservation quality of Bali bull’s spermatozoa. Selection for sperm quality was based on
sperm morphology. Cauda epididymis was obtained from slaughterhouse and the semen was collected and
examined with caffeine after cryopreservation. The spermatozoa were stained with Acredine Orange by
microscopy laser-scanning confocal microscope (BioRad MRC-1024). The result demonstrated that control
have high value of normal sperm cell than those treated with caffeine.
Key words: cauda epididymis, abnormality, caffeine |
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