Effect of Caffeine on Morphology of Epididymis Spermatozoa of Bali Bull

• Main Authors: Putranti, Oktora D, Soeparna, Lestari, Tita Damayanti, Adriani, Lovita
• Format: Book PeerReviewed
• Language: English; English; Indonesian
• Published: IPB Bogor 2015
• Subjects: SF600-1100 Veterinary medicine
• Online Access: http://repository.unair.ac.id/74082/2/Bukti%208%20Effect%20of%20Caffeine%20on%20Morphology%20of%20.......pdf; http://repository.unair.ac.id/74082/1/Bukti%208%20Effect%20of%20Caffeine%20on%20Morphology%20of%20Epididymis%20Spermatozoa%20of%20Bali%20Bull%20%282%29.pdf; http://repository.unair.ac.id/74082/3/Penilaian%208%20Effect%20of%20Caffeine%20on%20Morphology%20of%20.......pdf; http://repository.unair.ac.id/74082/; http://fapet.ipb.ac.id/~isai/pdf/ISAI_%202015_3_2015.pdf
• Institution: Universitas Airlangga

There is an unexpected loss of breeding animals of high genetic value for any reason, such as loss of libido, reproductive tract injury, or death. This loss can be reduced by harvesting spermatozoa from the epididymis with cryopreservation. However, after cryopreservation, there is still a decline of 50% in sperm post-thaw viability after thawing process. This research was conducted to evaluate the use of caffeine to improve cryopreservation quality of Bali bull’s spermatozoa. Selection for sperm quality was based on sperm morphology. Cauda epididymis was obtained from slaughterhouse and the semen was collected and examined with caffeine after cryopreservation. The spermatozoa were stained with Acredine Orange by microscopy laser-scanning confocal microscope (BioRad MRC-1024). The result demonstrated that control have high value of normal sperm cell than those treated with caffeine. Key words: cauda epididymis, abnormality, caffeine