The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats.

• Main Authors: Irma Santi, Budi Suprapti, Imam Susilo, NIDN. 0010086503, Junaidi Khotib, NIDN. 0022107001
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Badan Penelitian dan Pengembangan Kesehatan Kemenkes RI 2016
• Subjects: R Medicine (General); RB Pathology
• Online Access: http://repository.unair.ac.id/85852/1/The%20Effect%20of%20N-Acetyl-L-Cystein_compressed.pdf; http://repository.unair.ac.id/85852/2/Peer%20The%20Effect%20of%20N-Acetyl-L-Cysteine.pdf; http://repository.unair.ac.id/85852/3/13.%20Efek%20N-Asetil-L-Sistein%20%28NAC%29.pdf; http://repository.unair.ac.id/85852/
• Institution: Universitas Airlangga

Endothelial dysfunction is an early state of symptoms in a cardiovascular disease. An elevated oxidative stress plays a key role in the pathogenesis of macrovascular diabetic complication. The present study was design to evaluate the effect of NAC on plasma ADMA level and the expression of VCAM-1 protein on endothelial dysfunction in diabetic rats. Thirty male Sprague–Dawley rats were divided into 5 groups i.e. normal rats, diabetic rats, treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW. Diabetic rats model was induced by intraperitonial administration of alloxan monohydrate at dose of 150 mg/kgBW, diabetes occurred on 3nd day after alloxan injection and then started treatment of N-acetyl-L-cystein for 28 days. ADMA plasma level was analyzed with Elisa Reader and the expression of VCAM-1 protein was evaluated by immunohistochemistry. Conclusion of this research is that treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW for 28 days may prevent oxidative stress indicated by the decreasing of plasma ADMA level by 45.8%: 55.75% and 65.92%, respectively and the decreasing of the expression of VCAM-1 protein on endothelial dysfunction in diabetic rat by 24%: 31.75% and 58.92%, respectively.