The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats.
Endothelial dysfunction is an early state of symptoms in a cardiovascular disease. An elevated oxidative stress plays a key role in the pathogenesis of macrovascular diabetic complication. The present study was design to evaluate the effect of NAC on plasma ADMA level and the expression of VCAM-1...
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2016
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id-langga.858522019-08-20T03:49:32Z http://repository.unair.ac.id/85852/ The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. Irma Santi Budi Suprapti Imam Susilo, NIDN. 0010086503 Junaidi Khotib, NIDN. 0022107001 R Medicine (General) RB Pathology Endothelial dysfunction is an early state of symptoms in a cardiovascular disease. An elevated oxidative stress plays a key role in the pathogenesis of macrovascular diabetic complication. The present study was design to evaluate the effect of NAC on plasma ADMA level and the expression of VCAM-1 protein on endothelial dysfunction in diabetic rats. Thirty male Sprague–Dawley rats were divided into 5 groups i.e. normal rats, diabetic rats, treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW. Diabetic rats model was induced by intraperitonial administration of alloxan monohydrate at dose of 150 mg/kgBW, diabetes occurred on 3nd day after alloxan injection and then started treatment of N-acetyl-L-cystein for 28 days. ADMA plasma level was analyzed with Elisa Reader and the expression of VCAM-1 protein was evaluated by immunohistochemistry. Conclusion of this research is that treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW for 28 days may prevent oxidative stress indicated by the decreasing of plasma ADMA level by 45.8%: 55.75% and 65.92%, respectively and the decreasing of the expression of VCAM-1 protein on endothelial dysfunction in diabetic rat by 24%: 31.75% and 58.92%, respectively. Badan Penelitian dan Pengembangan Kesehatan Kemenkes RI 2016 Article PeerReviewed text en http://repository.unair.ac.id/85852/1/The%20Effect%20of%20N-Acetyl-L-Cystein_compressed.pdf text en http://repository.unair.ac.id/85852/2/Peer%20The%20Effect%20of%20N-Acetyl-L-Cysteine.pdf text en http://repository.unair.ac.id/85852/3/13.%20Efek%20N-Asetil-L-Sistein%20%28NAC%29.pdf Irma Santi and Budi Suprapti and Imam Susilo, NIDN. 0010086503 and Junaidi Khotib, NIDN. 0022107001 (2016) The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. Buletin Penelitian Kesehatan, 44 (3). pp. 147-152. ISSN 0125-9695 |
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R Medicine (General) RB Pathology Irma Santi Budi Suprapti Imam Susilo, NIDN. 0010086503 Junaidi Khotib, NIDN. 0022107001 The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. |
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Endothelial dysfunction is an early state of symptoms in
a cardiovascular disease. An elevated oxidative stress
plays a key role in the pathogenesis of macrovascular diabetic complication. The present study was design
to evaluate the effect of NAC on plasma ADMA level and the expression of VCAM-1 protein on endothelial dysfunction in diabetic rats. Thirty male Sprague–Dawley rats were divided into 5 groups i.e. normal rats, diabetic rats, treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW. Diabetic rats model was induced by intraperitonial administration of alloxan monohydrate at dose of 150 mg/kgBW, diabetes occurred on 3nd day after alloxan injection and then started treatment of N-acetyl-L-cystein for 28 days. ADMA plasma level was analyzed with Elisa Reader and the expression of VCAM-1 protein was evaluated by immunohistochemistry. Conclusion of this research is that treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and
NAC 100 mg/kgBW for 28 days may prevent oxidative stress indicated by the decreasing of plasma ADMA level by 45.8%: 55.75% and 65.92%, respectively and the decreasing of the expression of VCAM-1 protein on endothelial dysfunction in diabetic rat by 24%: 31.75% and 58.92%, respectively. |
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Article PeerReviewed |
author |
Irma Santi Budi Suprapti Imam Susilo, NIDN. 0010086503 Junaidi Khotib, NIDN. 0022107001 |
author_facet |
Irma Santi Budi Suprapti Imam Susilo, NIDN. 0010086503 Junaidi Khotib, NIDN. 0022107001 |
author_sort |
Irma Santi |
title |
The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. |
title_short |
The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. |
title_full |
The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. |
title_fullStr |
The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. |
title_full_unstemmed |
The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. |
title_sort |
effect of n-acetyl-l-cysteine (nac) on plasma adma level and the expression of vcam-1 protein on endotelial dysfunction in diabetic rats. |
publisher |
Badan Penelitian dan Pengembangan Kesehatan Kemenkes RI |
publishDate |
2016 |
url |
http://repository.unair.ac.id/85852/1/The%20Effect%20of%20N-Acetyl-L-Cystein_compressed.pdf http://repository.unair.ac.id/85852/2/Peer%20The%20Effect%20of%20N-Acetyl-L-Cysteine.pdf http://repository.unair.ac.id/85852/3/13.%20Efek%20N-Asetil-L-Sistein%20%28NAC%29.pdf http://repository.unair.ac.id/85852/ |
_version_ |
1681152100520689664 |