ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF

• Main Author: BUDI YANTI, NIM011417017321
• Format: Theses and Dissertations NonPeerReviewed
• Language: English; Indonesian; Indonesian; Indonesian
• Published: 2019
• Subjects: RC306-320.5 Tuberculosis
• Online Access: http://repository.unair.ac.id/85969/1/Dis.%20K.%2025-19%20Yan%20a%20ABSTRAK.pdf; http://repository.unair.ac.id/85969/2/Dis.%20K.%2025-19%20Yan%20a%20DAFTAR%20ISI.pdf; http://repository.unair.ac.id/85969/3/Dis.%20H.%2033-19%20Sul%20k%20DAFTAR%20PUSTAKA.pdf; http://repository.unair.ac.id/85969/4/Dis.%20K.%2025-19%20Yan%20a.pdf; http://repository.unair.ac.id/85969/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

Background: Hypervirulent strains of Mycobacterium tuberculosis are able to inhibit apoptosis and trigger necroptosis macrophage. Unlike necrosis, necroptosis has the kinetics of death through extrinsic and intrinsic signalling cell. Objective: To analyze developping necroptosis macrophage in the infection of hypervirulent strains of Mycobacterium tuberculosis in pulmonary TB patients. Methods: Mycobacterium tuberculosis isolate was taken from Bronchoalveolar lavage (BAL) in active pulmonary TB patients using bronchoscopy. The PCR was carried out using primary targets of TbD1 and RD9 for DNA extraction from BAL. The strains identification was done by sequencing using BLAST. FADD and RIP3 expressions were assessed by immunocitochemistry. The TNFα level was measured by ELISA sandwich indirect. Results: This study obtained thirty new and active pulmonary TB patients, 24 isolates of the hypervirulent homologous of Beijing Strains and 6 isolates of the M. bovis BCG homologous strain. The sample number 6 shows genetic variations such as deletion base A the nucleotide sequence number 277, this sequence is different from the referrens strain Beijing. Women were more infected with tuberculosis. Age ranges from 21-40 were more found to be infected. Laborer was the most found occupation. Apoptosis macrophage and FADD were be lower in hypervirulent strains of Mycobacterium tuberculosis infection (p <0.05). RIP3 expression was be lower in hypervirulent strain infections (p>0.05). The TNFα level was be lower in hypervirulent strains of Mycobacterium tuberculosis (p>0.05). Conclusion: In Surabaya, a hypervirulent strain of homologous Beijing strain and a non-virulent strain of homologous M. bovis were found. Macrophage necroptosis developped in tuberculosis patients could be used as the way to treat tuberculosis in the future through necroptotic pathways.