ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF
Background: Hypervirulent strains of Mycobacterium tuberculosis are able to inhibit apoptosis and trigger necroptosis macrophage. Unlike necrosis, necroptosis has the kinetics of death through extrinsic and intrinsic signalling cell. Objective: To analyze developping necroptosis macrophage in the...
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id-langga.859692019-08-20T11:44:36Z http://repository.unair.ac.id/85969/ ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF BUDI YANTI, NIM011417017321 RC306-320.5 Tuberculosis Background: Hypervirulent strains of Mycobacterium tuberculosis are able to inhibit apoptosis and trigger necroptosis macrophage. Unlike necrosis, necroptosis has the kinetics of death through extrinsic and intrinsic signalling cell. Objective: To analyze developping necroptosis macrophage in the infection of hypervirulent strains of Mycobacterium tuberculosis in pulmonary TB patients. Methods: Mycobacterium tuberculosis isolate was taken from Bronchoalveolar lavage (BAL) in active pulmonary TB patients using bronchoscopy. The PCR was carried out using primary targets of TbD1 and RD9 for DNA extraction from BAL. The strains identification was done by sequencing using BLAST. FADD and RIP3 expressions were assessed by immunocitochemistry. The TNFα level was measured by ELISA sandwich indirect. Results: This study obtained thirty new and active pulmonary TB patients, 24 isolates of the hypervirulent homologous of Beijing Strains and 6 isolates of the M. bovis BCG homologous strain. The sample number 6 shows genetic variations such as deletion base A the nucleotide sequence number 277, this sequence is different from the referrens strain Beijing. Women were more infected with tuberculosis. Age ranges from 21-40 were more found to be infected. Laborer was the most found occupation. Apoptosis macrophage and FADD were be lower in hypervirulent strains of Mycobacterium tuberculosis infection (p <0.05). RIP3 expression was be lower in hypervirulent strain infections (p>0.05). The TNFα level was be lower in hypervirulent strains of Mycobacterium tuberculosis (p>0.05). Conclusion: In Surabaya, a hypervirulent strain of homologous Beijing strain and a non-virulent strain of homologous M. bovis were found. Macrophage necroptosis developped in tuberculosis patients could be used as the way to treat tuberculosis in the future through necroptotic pathways. 2019 Thesis NonPeerReviewed text en http://repository.unair.ac.id/85969/1/Dis.%20K.%2025-19%20Yan%20a%20ABSTRAK.pdf text id http://repository.unair.ac.id/85969/2/Dis.%20K.%2025-19%20Yan%20a%20DAFTAR%20ISI.pdf text id http://repository.unair.ac.id/85969/3/Dis.%20H.%2033-19%20Sul%20k%20DAFTAR%20PUSTAKA.pdf text id http://repository.unair.ac.id/85969/4/Dis.%20K.%2025-19%20Yan%20a.pdf BUDI YANTI, NIM011417017321 (2019) ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF. Disertasi thesis, Universitas Airlangga. http://lib.unair.ac.id |
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RC306-320.5 Tuberculosis BUDI YANTI, NIM011417017321 ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF |
description |
Background: Hypervirulent strains of Mycobacterium tuberculosis are able to inhibit
apoptosis and trigger necroptosis macrophage. Unlike necrosis, necroptosis has the
kinetics of death through extrinsic and intrinsic signalling cell.
Objective: To analyze developping necroptosis macrophage in the infection of
hypervirulent strains of Mycobacterium tuberculosis in pulmonary TB patients.
Methods:
Mycobacterium tuberculosis isolate was taken from Bronchoalveolar lavage (BAL) in
active pulmonary TB patients using bronchoscopy. The PCR was carried out using
primary targets of TbD1 and RD9 for DNA extraction from BAL. The strains
identification was done by sequencing using BLAST. FADD and RIP3 expressions were
assessed by immunocitochemistry. The TNFα level was measured by ELISA sandwich
indirect.
Results:
This study obtained thirty new and active pulmonary TB patients, 24 isolates of the
hypervirulent homologous of Beijing Strains and 6 isolates of the M. bovis BCG
homologous strain. The sample number 6 shows genetic variations such as deletion
base A the nucleotide sequence number 277, this sequence is different from the
referrens strain Beijing. Women were more infected with tuberculosis. Age ranges from
21-40 were more found to be infected. Laborer was the most found occupation.
Apoptosis macrophage and FADD were be lower in hypervirulent strains of
Mycobacterium tuberculosis infection (p <0.05). RIP3 expression was be lower in
hypervirulent strain infections (p>0.05). The TNFα level was be lower in hypervirulent
strains of Mycobacterium tuberculosis (p>0.05).
Conclusion:
In Surabaya, a hypervirulent strain of homologous Beijing strain and a non-virulent
strain of homologous M. bovis were found. Macrophage necroptosis developped in
tuberculosis patients could be used as the way to treat tuberculosis in the future
through necroptotic pathways. |
format |
Theses and Dissertations NonPeerReviewed |
author |
BUDI YANTI, NIM011417017321 |
author_facet |
BUDI YANTI, NIM011417017321 |
author_sort |
BUDI YANTI, NIM011417017321 |
title |
ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF |
title_short |
ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF |
title_full |
ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF |
title_fullStr |
ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF |
title_full_unstemmed |
ANALISIS EKSPRESI FADD DAN RIP3 NEKROPTOSIS MAKROFAG PADA INFEKSI Mycobacterium tuberculosis GENOTIPE STRAIN HIPERVIRULEN PASIEN TB PARU AKTIF |
title_sort |
analisis ekspresi fadd dan rip3 nekroptosis makrofag pada infeksi mycobacterium tuberculosis genotipe strain hipervirulen pasien tb paru aktif |
publishDate |
2019 |
url |
http://repository.unair.ac.id/85969/1/Dis.%20K.%2025-19%20Yan%20a%20ABSTRAK.pdf http://repository.unair.ac.id/85969/2/Dis.%20K.%2025-19%20Yan%20a%20DAFTAR%20ISI.pdf http://repository.unair.ac.id/85969/3/Dis.%20H.%2033-19%20Sul%20k%20DAFTAR%20PUSTAKA.pdf http://repository.unair.ac.id/85969/4/Dis.%20K.%2025-19%20Yan%20a.pdf http://repository.unair.ac.id/85969/ http://lib.unair.ac.id |
_version_ |
1681152117502377984 |