Profile of Mycolic Acid Cleavage Products of Isoniazid Resistant Mycobacterium tuberculosis Isolate By Gas Chromatography-Mass Spectrometry
Mycolic acid cleavage products (MACPs) profile of Mycobacterium tuberculosis (MTB) which is resistant to isoniazid (INH) has been studied. This study describes the profile of MAs based on their cleavage products (MACPs) after esterification process, using BF3 in methanol as a catalyst, by gas chroma...
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| Main Authors: | , , , |
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| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
Dr. Yashwant Research Labs Pvt. Ltd
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/87223/6/C-11b.pdf http://repository.unair.ac.id/87223/1/Profile%20of%20Mycolic%20Acid%20Cleavage%20Products%20of%20Isoniazid%20Resistant%20Mycobacterium%20tuberculosis%20Isolate%20By%20Gas%20Chromatography-Mass%20Spectrometry%20%281%29.pdf http://repository.unair.ac.id/87223/5/Val%20C-11.pdf http://repository.unair.ac.id/87223/ https://ijpcr.com/volume8issue5suppl1/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Mycolic acid cleavage products (MACPs) profile of Mycobacterium tuberculosis (MTB) which is resistant to isoniazid (INH) has been studied. This study describes the profile of MAs based on their cleavage products (MACPs) after esterification process, using BF3 in methanol as a catalyst, by gas chromatography-mass spectrometry detector (GC-MSD). The MACPs profile of INH resistant MTB was compared to MACPs of H37Rv strain and MACPs of INH sensitive MTB isolate. Objective of this study was to obtain the characteristic profile of cellular fatty acid and MACPs of INH-resistant MTB isolate using GC-MS. Samples were cultured in Middlebrook 7H10 medium for more than 21 days. MAs and cellular fatty acids were extracted with chloroform prior to esterification for 1 hour at (90-95)o C using methanolic-BF3 as a catalyst. MACPs were extracted with heptane before injected into GC-MSD. This study obtained that the profile of six characteristic peaks of cellular fatty acids and MACPs of samples of INH-resistant MTB isolate was not significantly different to the INH-sensitive MTB isolate. But, the profile of H37Rv strain was significantly different compared to both INH-resistant MTB and INH-sensitive MTB isolate. |
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