In Vitro Effectivity Para Methoxy Cinnamate Acid (PMCA) In Solid Lipid Nanostructure (SLN) System Using Cetyl Alcohol As Lipid Formulated In HPC-H Gel Base

• Main Authors: Noorma Rosita, Widji Soeratri, Tristiana Erawati, Ayunanda, Wakhida Fitriyani
• Format: Article PeerReviewed
• Language: English; English; English; English; English; English; English; English; English
• Published: WJPR 2014
• Subjects: R Medicine; RS Pharmacy and materia medica
• Online Access: http://repository.unair.ac.id/87982/3/Artikel%20C-11.pdf; http://repository.unair.ac.id/87982/1/C-11%20Result%20-%20In%20Vitro%20Effectivity%20Para%20Methoxy%20Cinnamate%20Acid%20%28PMCA%29%20In%20Solid%20Lipid%20Nanostructure%20%28SLN%29%20System%20Using%20Cetyl%20Alcohol%20As%20Lipid%20Formulated%20In%20HPC-H%20Gel%20Base.pdf; http://repository.unair.ac.id/87982/4/C-11%20Reviewer.pdf; http://repository.unair.ac.id/87982/5/C-13%20Artikel.pdf; http://repository.unair.ac.id/87982/6/C-13%20Result.pdf; http://repository.unair.ac.id/87982/7/C-13%20Reviewer.pdf; http://repository.unair.ac.id/87982/10/Artikel%20C-14.pdf; http://repository.unair.ac.id/87982/11/Similarity%20C-14.pdf; http://repository.unair.ac.id/87982/12/Validasi%20C-14.pdf; http://repository.unair.ac.id/87982/; https://wjpr.net/download/article/1405423328.pdf
• Institution: Universitas Airlangga

Introduction: In vitro effectivity of solid lipid nanostructure (SLN) as carrier system has been studied on para methoxy cinnamate acid (PMCA) as a means of releasing PMCA and penetrating to prolong antiinflamasi preparation. Methods: SLN was made using cetyl alcohol as lipid and Tween 80 as stabilizer, produced by high shear homogenisatian (HPH) method. SLN was then formulated in HPC-H 3% gel base. Release study were conducted in phosphat buffer medium pH 7,4±0,05 at 32°C and stirred at 100 rpm using apparatus type 5-paddle overdisk. Penetration study were investigated in same medium at 37°C by using Wistar male rat skin 2-3 month age and weight 115-180 gram, as a membrane. Results: Both PMCA released and penetrated PMCA concentration was measured spectrophotometrically at 286 nm as λ max. Spherical SLNAPMS with particle size of 26-265 nm and 64.86% drug entrapment were produced. PMCASLN had pH 3,79±0,05 in HPC-H gel. In vitro release and penetration study were investigated using PMCA only (F-I), PMCA in macroemulsion (F-II) and PMCA-SLN (F-III) which was formulated in gel HPC-H. PMCA which was dispersed in gel had largest release and higher penetration, followed by PMCA in macroemulsion and lastly PMCA-SLN. Release flux for formula PMCA only, PMCA in Macroemulsion and PMCA-SLN were 62,6969 ± 1,33; 48,7949 ± 1,07 and 27,4652 ±0,84 µg/cm2/second-½ , whereas penteration flux were 0,3065 ± 0,1470; 0,1795 ± 0,0634 and 0,2925 ± 0,0996 µg/cm2/second1 for formula I, II and III respectively. Conclusion: SLN system has been able to retard released and penetrated PMCA.