Validasi metode kromatografi gas-spektrometri massa untuk penetapan kadar residu endosulfan dalam kubis

• Main Authors: Dini Tri Anggraini, Riesta Primaharinastiti, Isnaeni
• Format: Article PeerReviewed
• Language: English; English
• Published: Fakultas Farmasi Universitas Airlangga 2013
• Subjects: R Medicine; RS Pharmacy and materia medica
• Online Access: http://repository.unair.ac.id/90519/1/C-27b.pdf; http://repository.unair.ac.id/90519/3/Val%20C-27.pdf; http://repository.unair.ac.id/90519/; http://journal.unair.ac.id/BIKF@validasi-metode-kromatografi-gas-spektrometri-massa-untuk-penetapan-kadar-residu-endosulfan--dalam-kubis-article-6438-media-96-category-15.html
• Institution: Universitas Airlangga

The purpose of this study is to develop and validate a simple method suitable for analysis of trace amounts of endosulfan in cabbage. Cabbage samples were extracted with acetone and endosulfan was partitioned into dichloromethane/n-hexane (1:1, v/v). Final determination was performed by gas chromatography with mass spectrometry. Recovery studies were performed at 0.02 mg/kg fortification level of each compound (endosulfan I, endosulfan II, endosulfan sulfat) and the recoveries obtained ranged 69.67% to 124.02% with coefficient of variation values of 5.56-11.54%. The method showed good linearity over the range assayed 2-6 µg/mL and the detection and quantification limits for endosulfan studied varied, 0.1273 µg/mL to 0.1469 µg/mL and 0.856 µg/mL to 0.4451 µg/mL respectively.