VALIDASI METODE KROMATOGRAFI GAS DETEKTOR SPEKTROMETRI MASSA UNTUK PENETAPAN KADAR VITAMIN D3 DALAM SEDIAAN TABLET MULTIVITAMIN
Vitamin D3 is a fat-soluble vitamin that is available with other vitamins in multivitamin tablet. As a health supplement, the amount of vitamin D3 in multivitamin tablet is 400 IU or equivalent to 10μg in each tablet. Due to the low level of vitamin D3 contained in this complex sample matrix, a...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English English Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/90863/1/FF.KF.17-19%20Mur%20v%20abstrak.pdf http://repository.unair.ac.id/90863/2/FF.KF.17-19%20Mur%20v%20daftar%20isi.pdf http://repository.unair.ac.id/90863/3/FF.KF.17-19%20Mur%20v%20daftar%20pustaka.pdf http://repository.unair.ac.id/90863/4/FF.KF.17-19%20Mur%20v.pdf http://repository.unair.ac.id/90863/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English Indonesian |
| Summary: | Vitamin D3 is a fat-soluble vitamin that is available with other vitamins in
multivitamin tablet. As a health supplement, the amount of vitamin D3 in
multivitamin tablet is 400 IU or equivalent to 10μg in each tablet. Due to
the low level of vitamin D3 contained in this complex sample matrix, a
specific and sensitive method is needed to ensure the product quality, safety
and efficacy. Therefore, Gas Chromatography – Mass Spectrometry is used
in this study, because of its high sensitivity, high resolution, andspecifically
detection ofthe analytesat low concentration in samples. The objective of
the present study was to validate the GC-MS methods in terms of
specificity, linearity, range, accuracy and precision. The proposed method
was carried out without derivatization. The optimum condition was reached
by oven temperature program with initial temperature of 150°C for 2
minutes, then increased by 25°C/minute to the final temperature of 280°C
for 10 minutes. The time needed for running is about 17.20 minutes. From
the result of the method validation, the retention time of vitamin D3 was 8
minutes with resolution of 2.86. The method showed a linear response
(r=0.997) with Vxo of 3.54%. The average of percent recovery of the
sample was 98.71% with relative standard deviation of 2.57%. Detection
and quantitation limits were found to be 0.61 ppm and 1.85 ppm,
respectively. |
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