PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI
Black tea contains EGCG and caffeine which have pharmacological effect. The concentration of EGCG and caffeine is not informed in the label of black tea products. This study aims to determine the concentration of EGCG and caffeine simultaneously in local black tea products using thin layer chrom...
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id-langga.909022019-11-14T05:19:57Z http://repository.unair.ac.id/90902/ PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI INDY BUSTANUL HAQ, 051511133125 RS Pharmacy and materia medica Black tea contains EGCG and caffeine which have pharmacological effect. The concentration of EGCG and caffeine is not informed in the label of black tea products. This study aims to determine the concentration of EGCG and caffeine simultaneously in local black tea products using thin layer chromatography (TLC)-densitometry. Sample preparation was carried out by extraction of the powder of black tea product using 40 ml water at 80oC for 40 minutes with stirring and followed by extraction using ethyl acetate. The selected mobile phase was a mixture of chloroform: ethyl acetate: n-butanol: formic acid of (2:1:0.3:0.7) and the stationary phase was silica gel GF254. The resolution (Rs) among EGCG and caffeine with their nearest spots were 1.00 and 0.67. The selected wavelength for scanning analyt spots was 275 nm. The EGCG area was linear (r =0.9991; Vxo =3.02%) in the concentration range of 80-800 ppm. While caffeine was linear (r =0.9998; Vxo =0.89%) in the concentration range of 200-2000 ppm. The limit of detection and limit quantitation of EGCG and caffeine were 7.96 ppm, 26.52 ppm and 5.59 ppm, 18.62 ppm, respectively. The accuracy of EGCG and caffeine using bakud addition method were 99.44±7.83% and 96.59±5.22%, respectively. The values of validation parameter indicated that TLC-densitometry method can be used for determination of EGCG and caffeine simultaneously. The concentration of EGCG and caffeine in sample coded V, W, X, Y, Z were 0.17 ± 0.00%; 0.12 ± 0.00%; 0.15 ± 0.00%; 0.13 ± 0.01%; 0.10 ± 0.01% and 0.35 ± 0.03%; 0.35 ± 0.01% ; 0.54 ± 0.05%; 0.49 ± 0.05%; 0.56 ± 0.01% respectively.All samples were analyzed under conditions as received by consumers. 2019 Thesis NonPeerReviewed text en http://repository.unair.ac.id/90902/1/FF.KF.33-19%20Haq%20p%20abstrak.pdf text en http://repository.unair.ac.id/90902/2/FF.KF.33-19%20Haq%20p%20dafatr%20pustaka.pdf text en http://repository.unair.ac.id/90902/3/FF.KF.33-19%20Haq%20p%20daftar%20isi.pdf text id http://repository.unair.ac.id/90902/4/FF.KF.33-19%20Haq%20p.pdf INDY BUSTANUL HAQ, 051511133125 (2019) PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI. Skripsi thesis, Universitas Airlangga. Http://lib.Unair.ac.id |
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RS Pharmacy and materia medica INDY BUSTANUL HAQ, 051511133125 PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI |
description |
Black tea contains EGCG and caffeine which have
pharmacological effect. The concentration of EGCG and caffeine is not
informed in the label of black tea products. This study aims to determine the
concentration of EGCG and caffeine simultaneously in local black tea
products using thin layer chromatography (TLC)-densitometry. Sample
preparation was carried out by extraction of the powder of black tea product
using 40 ml water at 80oC for 40 minutes with stirring and followed by
extraction using ethyl acetate. The selected mobile phase was a mixture of
chloroform: ethyl acetate: n-butanol: formic acid of (2:1:0.3:0.7) and the
stationary phase was silica gel GF254. The resolution (Rs) among EGCG and
caffeine with their nearest spots were 1.00 and 0.67. The selected
wavelength for scanning analyt spots was 275 nm. The EGCG area was
linear (r =0.9991; Vxo =3.02%) in the concentration range of 80-800 ppm.
While caffeine was linear (r =0.9998; Vxo =0.89%) in the concentration
range of 200-2000 ppm. The limit of detection and limit quantitation of
EGCG and caffeine were 7.96 ppm, 26.52 ppm and 5.59 ppm, 18.62 ppm,
respectively. The accuracy of EGCG and caffeine using bakud addition
method were 99.44±7.83% and 96.59±5.22%, respectively. The values of
validation parameter indicated that TLC-densitometry method can be used
for determination of EGCG and caffeine simultaneously. The concentration
of EGCG and caffeine in sample coded V, W, X, Y, Z were 0.17 ± 0.00%;
0.12 ± 0.00%; 0.15 ± 0.00%; 0.13 ± 0.01%; 0.10 ± 0.01% and 0.35 ±
0.03%; 0.35 ± 0.01% ; 0.54 ± 0.05%; 0.49 ± 0.05%; 0.56 ± 0.01%
respectively.All samples were analyzed under conditions as received by
consumers. |
format |
Theses and Dissertations NonPeerReviewed |
author |
INDY BUSTANUL HAQ, 051511133125 |
author_facet |
INDY BUSTANUL HAQ, 051511133125 |
author_sort |
INDY BUSTANUL HAQ, 051511133125 |
title |
PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM
PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI |
title_short |
PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM
PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI |
title_full |
PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM
PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI |
title_fullStr |
PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM
PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI |
title_full_unstemmed |
PENENTUAN KADAR EPIGALOKATEKIN GALAT(EGCG) DAN KOFEIN DALAM
PRODUK TEH HITAM DOMESTIK DENGAN METODE KLT-DENSITOMETRI |
title_sort |
penentuan kadar epigalokatekin galat(egcg) dan kofein dalam
produk teh hitam domestik dengan metode klt-densitometri |
publishDate |
2019 |
url |
http://repository.unair.ac.id/90902/1/FF.KF.33-19%20Haq%20p%20abstrak.pdf http://repository.unair.ac.id/90902/2/FF.KF.33-19%20Haq%20p%20dafatr%20pustaka.pdf http://repository.unair.ac.id/90902/3/FF.KF.33-19%20Haq%20p%20daftar%20isi.pdf http://repository.unair.ac.id/90902/4/FF.KF.33-19%20Haq%20p.pdf http://repository.unair.ac.id/90902/ |
_version_ |
1681152904833007616 |