PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium)

• Main Author: NURITA TRI WIJAYANTI, NIM01151801603
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian; Indonesian; Indonesian
• Published: 2019
• Subjects: RE Ophthalmology
• Online Access: http://repository.unair.ac.id/92470/1/PPDS.IKM.%2015-19%20Wij%20p%20ABSTRAK.pdf; http://repository.unair.ac.id/92470/2/PPDS.IKM.%2015-19%20Wij%20p%20DAFTAR%20ISI.pdf; http://repository.unair.ac.id/92470/3/PPDS.IKM.%2015-19%20Wij%20p%20DAFTAR%20PUSTAKA.pdf; http://repository.unair.ac.id/92470/4/PPDS.IKM.%2015-19%20Wij%20p%20BR.pdf; http://repository.unair.ac.id/92470/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

Purpose : investigate antifibrotic effect of FG and LMSCs-CM on expression of Col-1, MMP-3, and TIMP-2 in HTFs of glaucomatous eye. Methods : HTF’s from glaucomatous eyes divided into 4 groups consist of FBS 2% control group, MMC (Mitomycin C), Fibrin Glue (FG) and LMSCs-CM treated group. The experiment was conducted in 7 days. Col-1, MMP-3, and TIMP-2 expression were assessed with immunofluorescence staining. Intensity of fluorescence was measured using ImageJ software. Difference of expression level among groups were analyzed using Oneway ANOVA test followed by post-hoc test with p<0.05 considered to be significant Results : Col-1 expression were decreased FG (0.78 ± 0.09 pixels) and LMSCs- CM conditioned media (0.78 ± 0.10 pixels) compared to the FBS 2% control group (1.52 ± 0.33 pixels) (p = 0,000). However, MMC provides better antibrotic effects on HTFs revealed with decreased of Col-1 expresion in MMC group (0.41 ± 0.28 pixels). MMP-3 expression levels were found to be increased in the MMC group (1.87 ± 0.58 pixels), FG (1.67 ± 0.48 pixels), and LMSCs-CM (1.58 ± 0.56 pixels) compared to the FBS 2% control group (0.90 ± 0.11 pixels) (p = 0.002). A decrease in the expression of TIMP-2 showed significant results in the MMC group (0.72 ± 0.31 pixel), FG (0.73 ± 0.40 pixel) and LMSCs-CM (0.80 ± 0.23 pixel) compared to the PBS control group of 2% (1.36 ± 0.36 pixels) (p = 0.002). Conclusion : FG and and LMSCs-CM might have antifibrotic effect on HTFs through modulation of extracellular matrix