PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium)
Purpose : investigate antifibrotic effect of FG and LMSCs-CM on expression of Col-1, MMP-3, and TIMP-2 in HTFs of glaucomatous eye. Methods : HTF’s from glaucomatous eyes divided into 4 groups consist of FBS 2% control group, MMC (Mitomycin C), Fibrin Glue (FG) and LMSCs-CM treated group. The ex...
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id-langga.924702019-12-11T02:42:43Z http://repository.unair.ac.id/92470/ PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) NURITA TRI WIJAYANTI, NIM01151801603 RE Ophthalmology Purpose : investigate antifibrotic effect of FG and LMSCs-CM on expression of Col-1, MMP-3, and TIMP-2 in HTFs of glaucomatous eye. Methods : HTF’s from glaucomatous eyes divided into 4 groups consist of FBS 2% control group, MMC (Mitomycin C), Fibrin Glue (FG) and LMSCs-CM treated group. The experiment was conducted in 7 days. Col-1, MMP-3, and TIMP-2 expression were assessed with immunofluorescence staining. Intensity of fluorescence was measured using ImageJ software. Difference of expression level among groups were analyzed using Oneway ANOVA test followed by post-hoc test with p<0.05 considered to be significant Results : Col-1 expression were decreased FG (0.78 ± 0.09 pixels) and LMSCs- CM conditioned media (0.78 ± 0.10 pixels) compared to the FBS 2% control group (1.52 ± 0.33 pixels) (p = 0,000). However, MMC provides better antibrotic effects on HTFs revealed with decreased of Col-1 expresion in MMC group (0.41 ± 0.28 pixels). MMP-3 expression levels were found to be increased in the MMC group (1.87 ± 0.58 pixels), FG (1.67 ± 0.48 pixels), and LMSCs-CM (1.58 ± 0.56 pixels) compared to the FBS 2% control group (0.90 ± 0.11 pixels) (p = 0.002). A decrease in the expression of TIMP-2 showed significant results in the MMC group (0.72 ± 0.31 pixel), FG (0.73 ± 0.40 pixel) and LMSCs-CM (0.80 ± 0.23 pixel) compared to the PBS control group of 2% (1.36 ± 0.36 pixels) (p = 0.002). Conclusion : FG and and LMSCs-CM might have antifibrotic effect on HTFs through modulation of extracellular matrix 2019 Thesis NonPeerReviewed text id http://repository.unair.ac.id/92470/1/PPDS.IKM.%2015-19%20Wij%20p%20ABSTRAK.pdf text id http://repository.unair.ac.id/92470/2/PPDS.IKM.%2015-19%20Wij%20p%20DAFTAR%20ISI.pdf text id http://repository.unair.ac.id/92470/3/PPDS.IKM.%2015-19%20Wij%20p%20DAFTAR%20PUSTAKA.pdf text id http://repository.unair.ac.id/92470/4/PPDS.IKM.%2015-19%20Wij%20p%20BR.pdf NURITA TRI WIJAYANTI, NIM01151801603 (2019) PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium). Thesis thesis, Universitas Airlangga. http://lib.unair.ac.id |
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RE Ophthalmology NURITA TRI WIJAYANTI, NIM01151801603 PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) |
description |
Purpose : investigate antifibrotic effect of FG and LMSCs-CM on expression of
Col-1, MMP-3, and TIMP-2 in HTFs of glaucomatous eye.
Methods : HTF’s from glaucomatous eyes divided into 4 groups consist of FBS
2% control group, MMC (Mitomycin C), Fibrin Glue (FG) and LMSCs-CM
treated group. The experiment was conducted in 7 days. Col-1, MMP-3, and
TIMP-2 expression were assessed with immunofluorescence staining. Intensity of
fluorescence was measured using ImageJ software. Difference of expression level
among groups were analyzed using Oneway ANOVA test followed by post-hoc
test with p<0.05 considered to be significant
Results : Col-1 expression were decreased FG (0.78 ± 0.09 pixels) and LMSCs-
CM conditioned media (0.78 ± 0.10 pixels) compared to the FBS 2% control
group (1.52 ± 0.33 pixels) (p = 0,000). However, MMC provides better antibrotic
effects on HTFs revealed with decreased of Col-1 expresion in MMC group (0.41
± 0.28 pixels). MMP-3 expression levels were found to be increased in the MMC
group (1.87 ± 0.58 pixels), FG (1.67 ± 0.48 pixels), and LMSCs-CM (1.58 ± 0.56
pixels) compared to the FBS 2% control group (0.90 ± 0.11 pixels) (p = 0.002). A
decrease in the expression of TIMP-2 showed significant results in the MMC
group (0.72 ± 0.31 pixel), FG (0.73 ± 0.40 pixel) and LMSCs-CM (0.80 ± 0.23
pixel) compared to the PBS control group of 2% (1.36 ± 0.36 pixels) (p = 0.002).
Conclusion : FG and and LMSCs-CM might have antifibrotic effect on HTFs
through modulation of extracellular matrix |
format |
Theses and Dissertations NonPeerReviewed |
author |
NURITA TRI WIJAYANTI, NIM01151801603 |
author_facet |
NURITA TRI WIJAYANTI, NIM01151801603 |
author_sort |
NURITA TRI WIJAYANTI, NIM01151801603 |
title |
PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) |
title_short |
PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) |
title_full |
PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) |
title_fullStr |
PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) |
title_full_unstemmed |
PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) |
title_sort |
pengaruh fibrin glue dan media terkondisi sel punca mesenkimal limbus terhadap ekspresi collagen-1 (col-1), matrix metalloproteinase-3 (mmp-3), dan tissue inhibitor of metalloproteinase-2(timp-2) pada sel fibroblas tenon sebagai model penyembuhan luka pascatrabekulektomi in vitro (studi eksperimental laboratorium) |
publishDate |
2019 |
url |
http://repository.unair.ac.id/92470/1/PPDS.IKM.%2015-19%20Wij%20p%20ABSTRAK.pdf http://repository.unair.ac.id/92470/2/PPDS.IKM.%2015-19%20Wij%20p%20DAFTAR%20ISI.pdf http://repository.unair.ac.id/92470/3/PPDS.IKM.%2015-19%20Wij%20p%20DAFTAR%20PUSTAKA.pdf http://repository.unair.ac.id/92470/4/PPDS.IKM.%2015-19%20Wij%20p%20BR.pdf http://repository.unair.ac.id/92470/ http://lib.unair.ac.id |
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