Identification and Characterization Indigenous of Lactobacillus sp from Bovine Rumen Fluid of Slaughterhouse
The discovery and characterisation of indigenous lactic acid bacteria (LAB) are important for diversity microbes as candidate probiotic. This research was aimed to identify lactic acid bacteria isolate from isolation process of local bovine rumen fluid from slaughterhouse in Surabaya Indonesia. G...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item PeerReviewed |
| Language: | English English English |
| Published: |
Fakultas Kedokteran Hewan Universitas Airlangga Surabaya
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/93012/4/Prosiding%2013%20-%20Identification%20and%20Characterization.pdf http://repository.unair.ac.id/93012/2/Form%20PR%2C%20Validasi%20Karya%20Ilmiah%20-%2013.pdf http://repository.unair.ac.id/93012/3/13.%20Identification%20and%20Characterization%20Indigenous%20of%20Lactobacillus%20sp.pdf http://repository.unair.ac.id/93012/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | The discovery and characterisation of indigenous lactic acid bacteria (LAB) are
important for diversity microbes as candidate probiotic. This research was aimed
to identify lactic acid bacteria isolate from isolation process of local bovine rumen
fluid from slaughterhouse in Surabaya Indonesia. Genotypic testing was conducted
by analyzing 16S rDNA and biochemical identification. DNA of sample isolate was
isolated and then amplified in vitro through the PCR method. Determination of
nucleotide sequence of 16S rDNA was performed with sequencing method. The
result of nucleotide sequence was than compared with GenBank database. The
BLAST was then applied to identify the phylogenetic tree. Based on the biochemical
characterization and nucleotide sequences, that isolate was identified as Lactobacillus
rhamnosus subsp TG15. The result of this research showed that L.rhamnosus subsp
TG15 showed viability bacteria in MRSA as control as much as 1.1 × 108 CFU/ml, mean
while in MRSA pH 2, L.rhamnosus subsp TG15 showed its viability as much as 9.3 ×
106 CFU/ml. Viability of isolate on bile tolerance 0.3% was 2.4 × 107 CFU/ml). Index
antagonist bacteria test on S.aureus showed inhibition diameter as much as 2.0 mm
and in antagonist test on E.coli as much as 2.5 mm. Based on the result, it could be
concluded that this research found a new strain of lactic acid bacteria, L.rhamnosus
subsp TG15 and that isolate has ability as the probiotic candidate. |
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