Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis

Mycobacterium tuberculosis secreted proteins in culture filtrate and early phase of infection, such as early secretory antigen target 6 (ESAT6), culture filtrate protein 10 (CFP-10), and antigen 85 complex i.e. Ag85A, Ag85B, and Ag85C which played roles in adherence, invasion, cytolysis, and evading...

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Main Authors: Ni Made Mertaniasih, Didik Handijatno, Agnes Dwi Sis Perwitasari, Desak Nyoman Surya Suameitria Dewi, Much Zaenal Fanani, Ika Qurrotul Afifah
Format: Book Section PeerReviewed
Language:English
English
English
Published: Elsevier 2016
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Online Access:http://repository.unair.ac.id/94918/1/Sequence%20Analysis%20of%20The%20Gene%20Region%20.pdf
http://repository.unair.ac.id/94918/2/Sequence%20Analysis%20of%20The%20Gene%20Region%20Encoding%20ESAT-6%2C%20Ag85B%2C%20and%20Ag85C%20Proteins%20from%20Clinical%20Isolates%20of%20Mycobacterium%20tuberculosis.pdf
http://repository.unair.ac.id/94918/3/Sequence%20Analysis%20of%20The%20Gene%20Region%20Encoding%20ESAT-6%2C%20Ag85B%2C%20and%20Ag85C%20Proteins%20from%20Clinical%20Isolates%20of%20Mycobacterium%20tuberculosis.pdf
http://repository.unair.ac.id/94918/
https://www.sciencedirect.com/science/article/pii/S187661961600036X
https://doi.org/10.1016/j.proche.2016.01.035
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Institution: Universitas Airlangga
Language: English
English
English
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Summary:Mycobacterium tuberculosis secreted proteins in culture filtrate and early phase of infection, such as early secretory antigen target 6 (ESAT6), culture filtrate protein 10 (CFP-10), and antigen 85 complex i.e. Ag85A, Ag85B, and Ag85C which played roles in adherence, invasion, cytolysis, and evading cytosol of macrophage, were virulence factors that determined the immune responses important on pathogenesis of Tuberculosis (TB), including granuloma formation or tissue that determine the degree of disease. The purpose of this research was to analyze the gene region sequence encoding ESAT-6, Ag85B, and Ag85C of Mycobacterium tuberculosis. Mycobacterium tuberculosis strain analyzed was taken from sputum of pulmonary TB patients in East Java, Indonesia. Sequenced DNA analyzed using GENETYX Ver.10. There were no SNPs both inside and outside epitope region of gene encoding ESAT-6, Ag85B, and Ag85C from clinical isolates of Mycobacterium tuberculosis. From this study, it could be concluded that the highly conserved gene region encoding ESAT-6, Ag85B, and Ag85C revealed no sequence polymorphism SNPs in epitope regions among Mycobacterium tuberculosis clinical isolates from sputum specimens of pulmonary TB patients.