Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis
Mycobacterium tuberculosis secreted proteins in culture filtrate and early phase of infection, such as early secretory antigen target 6 (ESAT6), culture filtrate protein 10 (CFP-10), and antigen 85 complex i.e. Ag85A, Ag85B, and Ag85C which played roles in adherence, invasion, cytolysis, and evading...
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id-langga.949182020-03-10T01:46:38Z http://repository.unair.ac.id/94918/ Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis Ni Made Mertaniasih Didik Handijatno Agnes Dwi Sis Perwitasari Desak Nyoman Surya Suameitria Dewi Much Zaenal Fanani Ika Qurrotul Afifah R Medicine (General) RZ Other systems of medicine Mycobacterium tuberculosis secreted proteins in culture filtrate and early phase of infection, such as early secretory antigen target 6 (ESAT6), culture filtrate protein 10 (CFP-10), and antigen 85 complex i.e. Ag85A, Ag85B, and Ag85C which played roles in adherence, invasion, cytolysis, and evading cytosol of macrophage, were virulence factors that determined the immune responses important on pathogenesis of Tuberculosis (TB), including granuloma formation or tissue that determine the degree of disease. The purpose of this research was to analyze the gene region sequence encoding ESAT-6, Ag85B, and Ag85C of Mycobacterium tuberculosis. Mycobacterium tuberculosis strain analyzed was taken from sputum of pulmonary TB patients in East Java, Indonesia. Sequenced DNA analyzed using GENETYX Ver.10. There were no SNPs both inside and outside epitope region of gene encoding ESAT-6, Ag85B, and Ag85C from clinical isolates of Mycobacterium tuberculosis. From this study, it could be concluded that the highly conserved gene region encoding ESAT-6, Ag85B, and Ag85C revealed no sequence polymorphism SNPs in epitope regions among Mycobacterium tuberculosis clinical isolates from sputum specimens of pulmonary TB patients. Elsevier 2016 Book Section PeerReviewed text en http://repository.unair.ac.id/94918/1/Sequence%20Analysis%20of%20The%20Gene%20Region%20.pdf text en http://repository.unair.ac.id/94918/2/Sequence%20Analysis%20of%20The%20Gene%20Region%20Encoding%20ESAT-6%2C%20Ag85B%2C%20and%20Ag85C%20Proteins%20from%20Clinical%20Isolates%20of%20Mycobacterium%20tuberculosis.pdf text en http://repository.unair.ac.id/94918/3/Sequence%20Analysis%20of%20The%20Gene%20Region%20Encoding%20ESAT-6%2C%20Ag85B%2C%20and%20Ag85C%20Proteins%20from%20Clinical%20Isolates%20of%20Mycobacterium%20tuberculosis.pdf Ni Made Mertaniasih and Didik Handijatno and Agnes Dwi Sis Perwitasari and Desak Nyoman Surya Suameitria Dewi and Much Zaenal Fanani and Ika Qurrotul Afifah (2016) Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis. In: Molecular and Cellular Life Sciences: Infectious Diseases, biochemistry and Structural Biology 2015 Conference, MCLS 2015. Procedia Chemistry, 18 . Elsevier, Surabaya, pp. 225-230. ISBN 9781510620708 https://www.sciencedirect.com/science/article/pii/S187661961600036X https://doi.org/10.1016/j.proche.2016.01.035 |
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R Medicine (General) RZ Other systems of medicine Ni Made Mertaniasih Didik Handijatno Agnes Dwi Sis Perwitasari Desak Nyoman Surya Suameitria Dewi Much Zaenal Fanani Ika Qurrotul Afifah Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis |
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Mycobacterium tuberculosis secreted proteins in culture filtrate and early phase of infection, such as early secretory antigen target 6 (ESAT6), culture filtrate protein 10 (CFP-10), and antigen 85 complex i.e. Ag85A, Ag85B, and Ag85C which played roles in adherence, invasion, cytolysis, and evading cytosol of macrophage, were virulence factors that determined the immune responses important on pathogenesis of Tuberculosis (TB), including granuloma formation or tissue that determine the degree of disease. The purpose of this research was to analyze the gene region sequence encoding ESAT-6, Ag85B, and Ag85C of Mycobacterium tuberculosis. Mycobacterium tuberculosis strain analyzed was taken from sputum of pulmonary TB patients in East Java, Indonesia. Sequenced DNA analyzed using GENETYX Ver.10. There were no SNPs both inside and outside epitope region of gene encoding ESAT-6, Ag85B, and Ag85C from clinical isolates of Mycobacterium tuberculosis. From this study, it could be concluded that the highly conserved gene region encoding ESAT-6, Ag85B, and Ag85C revealed no sequence polymorphism SNPs in epitope regions among Mycobacterium tuberculosis clinical isolates from sputum specimens of pulmonary TB patients. |
format |
Book Section PeerReviewed |
author |
Ni Made Mertaniasih Didik Handijatno Agnes Dwi Sis Perwitasari Desak Nyoman Surya Suameitria Dewi Much Zaenal Fanani Ika Qurrotul Afifah |
author_facet |
Ni Made Mertaniasih Didik Handijatno Agnes Dwi Sis Perwitasari Desak Nyoman Surya Suameitria Dewi Much Zaenal Fanani Ika Qurrotul Afifah |
author_sort |
Ni Made Mertaniasih |
title |
Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis |
title_short |
Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis |
title_full |
Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis |
title_fullStr |
Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis |
title_full_unstemmed |
Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis |
title_sort |
sequence analysis of the gene region encoding esat-6, ag85b, and ag85 c proteins from clinical isolates of mycobacterium tuberculosis |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://repository.unair.ac.id/94918/1/Sequence%20Analysis%20of%20The%20Gene%20Region%20.pdf http://repository.unair.ac.id/94918/2/Sequence%20Analysis%20of%20The%20Gene%20Region%20Encoding%20ESAT-6%2C%20Ag85B%2C%20and%20Ag85C%20Proteins%20from%20Clinical%20Isolates%20of%20Mycobacterium%20tuberculosis.pdf http://repository.unair.ac.id/94918/3/Sequence%20Analysis%20of%20The%20Gene%20Region%20Encoding%20ESAT-6%2C%20Ag85B%2C%20and%20Ag85C%20Proteins%20from%20Clinical%20Isolates%20of%20Mycobacterium%20tuberculosis.pdf http://repository.unair.ac.id/94918/ https://www.sciencedirect.com/science/article/pii/S187661961600036X https://doi.org/10.1016/j.proche.2016.01.035 |
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