Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study
Background: High concentrations of Co, Ni, and Cr in the blood serum of dental technicians are strongly associated with free radical formation. It has highly reactive properties that can cause further oxidation of molecule in the vicinity. Purpose: This study intended to investigate whether the Dent...
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id-langga.950642023-03-20T07:09:20Z https://repository.unair.ac.id/95064/ Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study Titiek Berniyanti, - Retno Palupi, - Indah Listiana Kriswandini, - Taufan Bramantoro, - Indira L. Putri, - R Medicine RK Dentistry Background: High concentrations of Co, Ni, and Cr in the blood serum of dental technicians are strongly associated with free radical formation. It has highly reactive properties that can cause further oxidation of molecule in the vicinity. Purpose: This study intended to investigate whether the Dental Technician occupational exposure of Co, Ni and Cr, could contribute to the high incidence of cancer. Methods: This was a cross-sectional study to dental technicians, performed after acccepting ethical clearance. Blood was sampled in 3 examinations for Co, Ni, Cr using Atomic Absorbance Spectrophotometry (AAS), MDA was examined with TBARS test, also 8 OHdG and wildtype p53 proteins determined by ELISA method. Results: Comparative statistical analysis, showing a significant difference (p < 0.05) between levels of Co, Ni, and Cr in exposed groups to the control group. But, not all variables was proven to be positively correlated, only with Cr, and Co, and negatively correlated with wild-type p53. Conclusion: MDA,8-OHdG and wildtype p53 can be used as genotoxic biomarkers in the metal exposed group, since they can accurately reflect the degree of Oxidative damage. Biomed Central 2020 Article PeerReviewed text en https://repository.unair.ac.id/95064/1/07.%20Suitability%20of%20MDA%2C%208-OHdG%20and%20Wild-type%20p53%20as%20genotoxic%20biomarkers.pdf text en https://repository.unair.ac.id/95064/2/7.Suitability%20of%20MDA%2C%208-OHdG%20and%20Wild%20Type%20p53%20as%20Genotoxic%20biomarker%20innetal%20%28Co%2CNi%20and%20Cr%29%20Exposed%20dental%20technicians%20a%20crposssectiona.pdf text id https://repository.unair.ac.id/95064/3/7.%20%20validasii%20per%20reviewer%20%28suitability%29.pdf text id https://repository.unair.ac.id/95064/6/suitabily.pdf text id https://repository.unair.ac.id/95064/7/13.%20Validasi_Suitability%20of%20MDA%2C%208-OHdG%20and%20wild-type%20p53.pdf text en https://repository.unair.ac.id/95064/9/Turnitin_Suitability%20of%20MDA%2C%208-OHdG.pdf text id https://repository.unair.ac.id/95064/11/12.%20Kualitas%20Karil_Suitability%20of%20MDA%2C%208-OHdG%20and%20wild-type.pdf text en https://repository.unair.ac.id/95064/13/12.suitability.pdf Titiek Berniyanti, - and Retno Palupi, - and Indah Listiana Kriswandini, - and Taufan Bramantoro, - and Indira L. Putri, - (2020) Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study. BMC Oral Health, 20 (65). pp. 1-12. ISSN 14726831 https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-020-1049-1 https://doi.org/10.1186/s12903-020-1049-1 |
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R Medicine RK Dentistry Titiek Berniyanti, - Retno Palupi, - Indah Listiana Kriswandini, - Taufan Bramantoro, - Indira L. Putri, - Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study |
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Background: High concentrations of Co, Ni, and Cr in the blood serum of dental technicians are strongly associated with free radical formation. It has highly reactive properties that can cause further oxidation of molecule in the vicinity. Purpose: This study intended to investigate whether the Dental Technician occupational exposure of Co, Ni and Cr, could contribute to the high incidence of cancer. Methods: This was a cross-sectional study to dental technicians, performed after acccepting ethical clearance. Blood was sampled in 3 examinations for Co, Ni, Cr using Atomic Absorbance Spectrophotometry (AAS), MDA was examined with TBARS test, also 8 OHdG and wildtype p53 proteins determined by ELISA method. Results: Comparative statistical analysis, showing a significant difference (p < 0.05) between levels of Co, Ni, and Cr in exposed groups to the control group. But, not all variables was proven to be positively correlated, only with Cr, and Co, and negatively correlated with wild-type p53. Conclusion: MDA,8-OHdG and wildtype p53 can be used as genotoxic biomarkers in the metal exposed group, since they can accurately reflect the degree of Oxidative damage. |
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Article PeerReviewed |
author |
Titiek Berniyanti, - Retno Palupi, - Indah Listiana Kriswandini, - Taufan Bramantoro, - Indira L. Putri, - |
author_facet |
Titiek Berniyanti, - Retno Palupi, - Indah Listiana Kriswandini, - Taufan Bramantoro, - Indira L. Putri, - |
author_sort |
Titiek Berniyanti, - |
title |
Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study |
title_short |
Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study |
title_full |
Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study |
title_fullStr |
Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study |
title_full_unstemmed |
Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co,Ni and Cr) exposed dental technicians: a cross-sectional study |
title_sort |
suitability of mda, 8-ohdg and wild-type p53 as genotoxic biomarkers in metal (co,ni and cr) exposed dental technicians: a cross-sectional study |
publisher |
Biomed Central |
publishDate |
2020 |
url |
https://repository.unair.ac.id/95064/1/07.%20Suitability%20of%20MDA%2C%208-OHdG%20and%20Wild-type%20p53%20as%20genotoxic%20biomarkers.pdf https://repository.unair.ac.id/95064/2/7.Suitability%20of%20MDA%2C%208-OHdG%20and%20Wild%20Type%20p53%20as%20Genotoxic%20biomarker%20innetal%20%28Co%2CNi%20and%20Cr%29%20Exposed%20dental%20technicians%20a%20crposssectiona.pdf https://repository.unair.ac.id/95064/3/7.%20%20validasii%20per%20reviewer%20%28suitability%29.pdf https://repository.unair.ac.id/95064/6/suitabily.pdf https://repository.unair.ac.id/95064/7/13.%20Validasi_Suitability%20of%20MDA%2C%208-OHdG%20and%20wild-type%20p53.pdf https://repository.unair.ac.id/95064/9/Turnitin_Suitability%20of%20MDA%2C%208-OHdG.pdf https://repository.unair.ac.id/95064/11/12.%20Kualitas%20Karil_Suitability%20of%20MDA%2C%208-OHdG%20and%20wild-type.pdf https://repository.unair.ac.id/95064/13/12.suitability.pdf https://repository.unair.ac.id/95064/ https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-020-1049-1 https://doi.org/10.1186/s12903-020-1049-1 |
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