Eksplorasi Protein Antigenik Leucocytozoon caulleryi sebagai Kit Diagnostik Leucocytozoonosis pada Ayam Broiler
The purpose of this research to explored antigenic proteins of Leucocytozoon caulleryi as a diagnostic kit candidate of Leucocytozoonosis to broiler chicken. Exploration of antigenic proteins were performed by many steps: identification of gametocyte L.caull eryi from blood examination, isolation...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | English English Indonesian English |
| Published: |
The Association of Indonesia Veterinarians (PDHI) and The Faculty of Veterinary Medicine, Airlangga University
2013
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| Online Access: | https://repository.unair.ac.id/99972/1/Bukti%20C%2015%20Eksplorasi%20protein%20antigenik%20..._.pdf https://repository.unair.ac.id/99972/7/C15_Peer_Review_Endang_Suprihati.pdf https://repository.unair.ac.id/99972/4/Eksplorasi%20Protein%20Antigenik%20Leucocytozoon%20caulleryi%20sebagai%20Kit%20Diagnostik%20Leucocytozoonosis%20pada%20Ayam%20Broiler_Turn%20It%20In.pdf https://repository.unair.ac.id/99972/11/Form_Penilaian_Karil_Bid_Ilmu.pdf https://repository.unair.ac.id/99972/ http://journal.unair.ac.id/MKH@media-kedokteran-hewan-media-1.html |
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| Institution: | Universitas Airlangga |
| Language: | English English Indonesian English |
| Summary: | The purpose of this research to explored antigenic proteins of Leucocytozoon caulleryi as a diagnostic kit candidate of Leucocytozoonosis to broiler chicken. Exploration of antigenic proteins were performed by many steps: identification of gametocyte L.caull eryi from blood examination, isolation of L.caull eryi schizont from chicken organs, fractionation of whole proteins of L.caull eryi schizont by SDS-PAGE assay, and characterization antigenic proteins of L.caulleryi schizont by Western Blot assay.
The results showed that the characterization of L.caulleryi schizont protein have been recognized by SDS - PAGE, have molecular weight of 114.7 kDa , 109.6 kDa , 80.5 kDa , 65.0 kDa , 55.6 kDa and 36.1 kDa respectively. Some protein bands that appear thicker seen in protein with a molecular weight of 109.6 kDa , 65.0 kDa and 55.6 kDa. Characterization by Western blot obtained two protein fractions showed thick bands with molecular weight are 109.6 kDa and 65.0 kDa , whereas the other 4 protein fractions do not appear or are not reacted with antibodies ( IgG) from infected chicken serum.
The conclusion of the research showed that L.caulleryi schizont has six protein fractions to molecular weight. They were 114.7 kDa , 109.6 kDa , 80.5 kDa , 65.0 kDa , 55.6 kDa and 36.1kDa respectively . Among those protein fractions, 109.6 kDa and 65.0 kDa were antigenic proteins that could be developed as diagnostic kit.
Keywords: Leucocytozoon caulleryi, antigenic proteins, broiler chicken |
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