Eksplorasi Protein Antigenik Leucocytozoon caulleryi sebagai Kit Diagnostik Leucocytozoonosis pada Ayam Broiler

• Main Authors: Nunuk Dyah Retno Lastuti, -, Endang Suprihati, -, Dony Chrismanto, -
• Format: Article PeerReviewed
• Language: English; English; Indonesian; English
• Published: The Association of Indonesia Veterinarians (PDHI) and The Faculty of Veterinary Medicine, Airlangga University 2013
• Subjects: SF481-507 Poultry. Eggs; SF811-909 Veterinary medicine of special organs, regions, and systems
• Online Access: https://repository.unair.ac.id/99972/1/Bukti%20C%2015%20Eksplorasi%20protein%20antigenik%20..._.pdf; https://repository.unair.ac.id/99972/7/C15_Peer_Review_Endang_Suprihati.pdf; https://repository.unair.ac.id/99972/4/Eksplorasi%20Protein%20Antigenik%20Leucocytozoon%20caulleryi%20sebagai%20Kit%20Diagnostik%20Leucocytozoonosis%20pada%20Ayam%20Broiler_Turn%20It%20In.pdf; https://repository.unair.ac.id/99972/11/Form_Penilaian_Karil_Bid_Ilmu.pdf; https://repository.unair.ac.id/99972/; http://journal.unair.ac.id/[e-mail]
• Institution: Universitas Airlangga

The purpose of this research to explored antigenic proteins of Leucocytozoon caulleryi as a diagnostic kit candidate of Leucocytozoonosis to broiler chicken. Exploration of antigenic proteins were performed by many steps: identification of gametocyte L.caull eryi from blood examination, isolation of L.caull eryi schizont from chicken organs, fractionation of whole proteins of L.caull eryi schizont by SDS-PAGE assay, and characterization antigenic proteins of L.caulleryi schizont by Western Blot assay. The results showed that the characterization of L.caulleryi schizont protein have been recognized by SDS - PAGE, have molecular weight of 114.7 kDa , 109.6 kDa , 80.5 kDa , 65.0 kDa , 55.6 kDa and 36.1 kDa respectively. Some protein bands that appear thicker seen in protein with a molecular weight of 109.6 kDa , 65.0 kDa and 55.6 kDa. Characterization by Western blot obtained two protein fractions showed thick bands with molecular weight are 109.6 kDa and 65.0 kDa , whereas the other 4 protein fractions do not appear or are not reacted with antibodies ( IgG) from infected chicken serum. The conclusion of the research showed that L.caulleryi schizont has six protein fractions to molecular weight. They were 114.7 kDa , 109.6 kDa , 80.5 kDa , 65.0 kDa , 55.6 kDa and 36.1kDa respectively . Among those protein fractions, 109.6 kDa and 65.0 kDa were antigenic proteins that could be developed as diagnostic kit. Keywords: Leucocytozoon caulleryi, antigenic proteins, broiler chicken