PENGARUH EKSTRAK BUAH MERAH (Pandanus conoideus Lamk) TERHADAP KADAR SITOKIN TNF-α IL-10 DAN ANGKA PARASITEMIA PADA MENCIT SWISS (Mus musculus L) YANG DI INFEKSI Plasmodium berghei

• Main Authors: , Zeth Roberth Felle, S.Kep, , Dr. dr. Mahardika Agus W., DTM & H,M.Kes
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/120045/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=60052
• Institution: Universitas Gadjah Mada

Background: Reseach on the effects of red fruit has been conducted with various result. In this study, the effect of red fruit extract (EBM) on the level of cytokines TNF-α, IL-10 and the parasitemia in swiss mice infected with Plasmodium berghei well beassessed. Methods: The study used a laboratory quasy-experimental design with pre and post test only control group. Sixty male Swiss mice of 8 weeks old and weighs 20-30 g, was simply randomized into four treatment groups. Group I (K1) was stimulated with EBM for 2 weeks before and 2 weeks after infection with P.berghei. K2 was stimulated with EBM for 2 weeks before infection, K3 was stimulated with EBM for 2 weeks after infection and K4 is negative control was given 0.6% tween 40. The dose of EBM was 7.8 mg/30 g BW mice / po once a day. Serum level of TNF-α and IL-10, was measured by ELISA Sandwich methods and the number of parasitemia were examined microscopically. The differences levels of TNF-α, IL-10 and parasitemia of each treatment group were analyzed by t-test, one way anova, honestly significant different (HSD) and also multivariate analysis (manova). Results : There are significances differences number of parasitemia in treatment group was stimulated with EBM for 2 weeks after infection, if compared with negative control group, for day-6 is 13,735% and day-9 is 1,054%, and also to the treatment group was stimulated with EBM for 2 weeks before infection, which day-6 is 13,141% and day-9 is 32,455%. But negative control group shows, day-6 is 58,180 % and day-9 is 78,506%. The significances differences to the levels of TNF-α, showed also by treatment group was stimulated with EBM for 2 weeks after infection, which at day-3 is 23,269 pg/ml and day-6, is 22,244 pg/ml. But the negative control shows at day-3 is 69,983 pg/ml and day-9 is 94,679pg/ml. While all treatment group shown increase the levels of IL-10, and not significances differences if compared with the negative control. But the levels of IL-10 from treatment group was stimulated with EBM for 2 weeks after infection, has rise sharply, increase progressively by 252,892pg/ml. It is the highest concentration compared with other treatmen group. The increase levels of IL-10 from treatment group was stimulated with EBM for 2 weeks after infection, with the significantly reduces of parasitemia and the levels of TNF-α. But the increased levels of TNF-α is fatal evident, because with the death of mice, such as showed by treatment group was given EBM for 2 weeks before and 2 weeks after infection and treantemnt group was given EBM only 2 weeks before infection. Conclusion: The extract of red fruit can reduce the levels of TNF-α and the significantly progrees can increase the levels of IL-10. Red fruit extracts can also significantly reduce the number of parasitemia. The effects of EBM is more significant, especially when administered after malaria infection occurred.