FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL
Aphthous stomatitis is an oral wound which can be worsened due to food mastication and talking. Gamavuton-0 (GVT-0) a curcumin analogue which has an anti inflammatory activity. The purposes of this study were to develop and optimize formulations of GVT-0 buccoadhesive film using chitosan and PVP K-3...
محفوظ في:
المؤلفون الرئيسيون: | , |
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التنسيق: | Theses and Dissertations NonPeerReviewed |
منشور في: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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الوصول للمادة أونلاين: | https://repository.ugm.ac.id/123045/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63155 |
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id-ugm-repo.1230452016-03-04T08:32:08Z https://repository.ugm.ac.id/123045/ FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL , Dewa Ayu Arimurni , Dr.rer.nat. Ronny Martien, M.Si. ETD Aphthous stomatitis is an oral wound which can be worsened due to food mastication and talking. Gamavuton-0 (GVT-0) a curcumin analogue which has an anti inflammatory activity. The purposes of this study were to develop and optimize formulations of GVT-0 buccoadhesive film using chitosan and PVP K-30 and evaluate its effect on wound healing process based on numbers of polymorphonuclear cells in the rat buccal mucosa. Film formulas were determined by using simplex lattice design. Film�s physicochemical evaluations were done to determine the effect of chitosan and PVP K-30. The best obtained formula was used to study the transport of GVT-0, ex vivo residence time and its in-vivo anti inflammatory activity using negative control, placebo matrix control and treatment group. Each group consisted of 3 randomly selected male Wistar rats with the body weight range of 200-300 g which were sacrificed on days 1, 3, 5, and 7 post injury. The injury was made by biopsy punch (d=2.5mm). All film formulations were thin, flexible, smooth and transparent with weight and thickness uniformity. GVT-0 content in the films ranged from 91-100%. The GVT-0 release data fit well to the Higuchi expression and possesses suitable swelling profile and produce no irritation. The optimum ratio GVT-0 buccoadhesive film is 1.702% chitosan and 1.298% PVP K-30. In-vitro drug release from optimum formula showed that about 45.03±0.22% drug was released up to 6 hours and ex-vivo mucoadhesive time was found to be 125± 3.78 minutes. The GVT-0 permeation through rabbit buccal mucosa was found to be 2.30±0.125 for 6 hours. GVT-0 buccoadhesive film significantly can decrease the number of polymorphonuclear cells (p<0.05). In conclusion, buccoadhesive films of Gamavuton-0 can be proposed as a new therapeutic tool for aphthous stomatitis with drug was released locally and only a very small amount might have absorbed systemically. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Dewa Ayu Arimurni and , Dr.rer.nat. Ronny Martien, M.Si. (2013) FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63155 |
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ETD , Dewa Ayu Arimurni , Dr.rer.nat. Ronny Martien, M.Si. FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL |
description |
Aphthous stomatitis is an oral wound which can be worsened due to food mastication and talking. Gamavuton-0 (GVT-0) a curcumin analogue which has an anti inflammatory activity. The purposes of this study were to develop and optimize formulations of GVT-0 buccoadhesive film using chitosan and PVP K-30 and evaluate its effect on wound healing process based on numbers of polymorphonuclear cells in the rat buccal mucosa.
Film formulas were determined by using simplex lattice design. Film�s physicochemical evaluations were done to determine the effect of chitosan and PVP K-30. The best obtained formula was used to study the transport of GVT-0, ex vivo residence time and its in-vivo anti inflammatory activity using negative control, placebo matrix control and treatment group. Each group consisted of 3 randomly selected male Wistar rats with the body weight range of 200-300 g which were sacrificed on days 1, 3, 5, and 7 post injury. The injury was made by biopsy punch (d=2.5mm).
All film formulations were thin, flexible, smooth and transparent with weight and thickness uniformity. GVT-0 content in the films ranged from 91-100%. The GVT-0 release data fit well to the Higuchi expression and possesses suitable swelling profile and produce no irritation. The optimum ratio GVT-0 buccoadhesive film is 1.702% chitosan and 1.298% PVP K-30. In-vitro drug release from optimum formula showed that about 45.03±0.22% drug was released up to 6 hours and ex-vivo mucoadhesive time was found to be 125± 3.78 minutes. The GVT-0 permeation through rabbit buccal mucosa was found to be 2.30±0.125 for 6 hours. GVT-0 buccoadhesive film significantly can decrease the number of polymorphonuclear cells (p<0.05). In conclusion, buccoadhesive films of Gamavuton-0 can be proposed as a new therapeutic tool for aphthous stomatitis with drug was released locally and only a very small amount might have absorbed systemically. |
format |
Theses and Dissertations NonPeerReviewed |
author |
, Dewa Ayu Arimurni , Dr.rer.nat. Ronny Martien, M.Si. |
author_facet |
, Dewa Ayu Arimurni , Dr.rer.nat. Ronny Martien, M.Si. |
author_sort |
, Dewa Ayu Arimurni |
title |
FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL |
title_short |
FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL |
title_full |
FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL |
title_fullStr |
FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL |
title_full_unstemmed |
FORMULASI SEDIAAN FILM BUKOADHESIF GAMAVUTON-0 DENGAN MATRIKS KITOSAN DAN PVP K-30 SERTA PENGARUHNYA TERHADAP JUMLAH SEL LEUKOSIT POLIMORFONUKLEAR PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL |
title_sort |
formulasi sediaan film bukoadhesif gamavuton-0 dengan matriks kitosan dan pvp k-30 serta pengaruhnya terhadap jumlah sel leukosit polimorfonuklear pada proses penyembuhan luka mukosa bukal |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2013 |
url |
https://repository.ugm.ac.id/123045/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63155 |
_version_ |
1681231817270624256 |