ISOLASI, IDENTIFIKASI, DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP BAKTERI PENYEBAB INFEKSI YOLK SAC PADA ANAK AYAM PEDAGING KOMERSIAL

• Main Authors: , Hana Setyawati, , Prof. drh. Charles Rangga Tabbu, M.Sc., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123383/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63494
• Institution: Universitas Gadjah Mada

Yolk sac infection is the primary cause of chicks mortality in the first week of life. The disease is often accompanied by inflammation of the navel (omphalitis), and the chicks appear weak, drowsy, and anorexic. Egg contaminated by bacteria and unhealed navel may be the important route of transmission of the disease. Antibiotics are often used to treat yolk sac infection. However, the emergence of antibiotics resistant bacteria lead to more complicated disease healing process. The purposes of this study are to isolate and to identify yolk sac infectioncausing bacteria in commercial broiler chicks, and to evaluate the antibiotics susceptibility of these bacteria against commonly used antibiotics in the field to observe the most effective therapy for the treatment of yolk sac infection. Twentytwo, one to three days old broiler chicks with yolk sac infection were necropsied, and bacterial isolation was made from the yolk sac. Sample of yolk sac was inoculated in blood agar, followed by Gram stain and biochemical tests for identification of the isolated bacteria. Antibiotics susceptibility test was conducted based on Kirby-Bauer disc diffusion method to several antibiotics, including amoxcycillin-clavulanic acid, enrofloxacin, tetracycline, erythromycin, and colistin sulfate. Gram positive and Gram negative bacteri isolated were as much as 62% (21/34) and 38% (13/34), respectively. The most frequently Gram positive bacteria isolated was Enterococcus spp. (44.1% (15/34)), whereas the most frequently Gram negative bacteria isolated was Escherichia coli (11.8% (4/34)). Bacteria isolated from this study were Enterococcus spp. (44.1%), S. aureus (8.8%), Staphylococcus spp. (8.8%), E. coli (11.8%), Klebsiella sp. (8.8%), Serratia sp. (8.8%), and Gram negative non Enterobacteriaceae (8.8%). The majority of bacteria isolated were sensitive to amoxicillin-clavulanic acid (82.3%), tetracycline (41.2%), enrofloxacin (38.2%), and were resistant to colistin sulfate (100%), erythromycin (82.3%), tetracycline (55.9%), and enrofloxacin (38.2%). Based on the results of this experiment, it can be concluded that there were some sensitive and resistant bacteria isolated from infected yolk sac. Therefore, administration of antibiotic to commercial broiler should be given rationally in order to minimize the emergence of resistant bacteria which could prolong the disease healing process.