FENOTIPE DAN MOLEKULER Pasteurella multocida PENYEBAB BRONCHOPNEUMONIA PADA BABI DI YOGYAKARTA
Pasteurella multocida is an important species that are suspected to cause bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter house between 30-80%. Development of molecular techniques in support of diagnosis and analysis of genetic diversity by the method of Polymerase Chai...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Online Access: | https://repository.ugm.ac.id/123575/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63687 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Pasteurella multocida is an important species that are suspected to cause
bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter
house between 30-80%. Development of molecular techniques in support of
diagnosis and analysis of genetic diversity by the method of Polymerase Chain
Reaction (PCR) is needed. The purpose of this study were 1) determined the
microorganisms that caused respiratory disorders (cough) or pneumonia in pigs in
Yogyakarta area with morphological and biochemical tests, 2) confirmed the
results of identification using PCR and, 3) determined the genetic variation of P.
multocida isolates in Yogyakarta with other P. multocida isolates abroad. A
number of 6 pigs were obtained from some pig farming in Yogyakarta with
clinical symptoms of pneumonia were necropted, isolated and identified the cause
of pneumonia, followed by histopathological examination and biochemical
characterization of the isolates. Molecular test performed by DNA extraction
using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene
using forward primer 5'-GGA GTG AAC TGC AGC TAA TAC C-3', and reverse
primer 5'-GTA GGT AAG CTT CGC GTT GTT G-3', electrophoresis,
purification and sequencing. Macroscopic and histopathological examination
results were analyzed descriptively. Sequencing results were analyzed by multiple
alignment with other Pasteurella spp. taken from GenBank using the Clustal W
software, subsequently analyzed using Neighbour-Joining and Maximum
Parsimony method that exist in program MEGA version 5.1
The results showed that P. multocida is one of the main causes of
bronchopneumonia in pigs in Yogyakarta with clinical symptoms of anorexia,
dipsnoea, cough with serous to mucopurulent exudation. Pulmo showed grayish
hepatization on dorsocranial, multifocal hemorrhagic and congestion.
Histopathological changes found fibrinous bronchopneumonia and
bronchopneumonia catharrhalis. Analysis of 16S rRNA showed that P. multocida
isolates from lung of bronchopneumonia of swine in Yogyakarta with genetic
distance based on 765 nucleotides is at 0%. Filogram based on the nucleotide
sequence showed a high similarity between P. multocida isolates from Yogyakarta
and other isolates from USA, Germany, China, Europe, and Hungaria. |
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