FENOTIPE DAN MOLEKULER Pasteurella multocida PENYEBAB BRONCHOPNEUMONIA PADA BABI DI YOGYAKARTA

• Main Authors: , VICTOR LENDA, , Prof. drh. Kurniasih, MVSc. Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123575/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63687
• Institution: Universitas Gadjah Mada

Pasteurella multocida is an important species that are suspected to cause bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter house between 30-80%. Development of molecular techniques in support of diagnosis and analysis of genetic diversity by the method of Polymerase Chain Reaction (PCR) is needed. The purpose of this study were 1) determined the microorganisms that caused respiratory disorders (cough) or pneumonia in pigs in Yogyakarta area with morphological and biochemical tests, 2) confirmed the results of identification using PCR and, 3) determined the genetic variation of P. multocida isolates in Yogyakarta with other P. multocida isolates abroad. A number of 6 pigs were obtained from some pig farming in Yogyakarta with clinical symptoms of pneumonia were necropted, isolated and identified the cause of pneumonia, followed by histopathological examination and biochemical characterization of the isolates. Molecular test performed by DNA extraction using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene using forward primer 5'-GGA GTG AAC TGC AGC TAA TAC C-3', and reverse primer 5'-GTA GGT AAG CTT CGC GTT GTT G-3', electrophoresis, purification and sequencing. Macroscopic and histopathological examination results were analyzed descriptively. Sequencing results were analyzed by multiple alignment with other Pasteurella spp. taken from GenBank using the Clustal W software, subsequently analyzed using Neighbour-Joining and Maximum Parsimony method that exist in program MEGA version 5.1 The results showed that P. multocida is one of the main causes of bronchopneumonia in pigs in Yogyakarta with clinical symptoms of anorexia, dipsnoea, cough with serous to mucopurulent exudation. Pulmo showed grayish hepatization on dorsocranial, multifocal hemorrhagic and congestion. Histopathological changes found fibrinous bronchopneumonia and bronchopneumonia catharrhalis. Analysis of 16S rRNA showed that P. multocida isolates from lung of bronchopneumonia of swine in Yogyakarta with genetic distance based on 765 nucleotides is at 0%. Filogram based on the nucleotide sequence showed a high similarity between P. multocida isolates from Yogyakarta and other isolates from USA, Germany, China, Europe, and Hungaria.