IDENTIFIKASI GOLONGAN SENYAWA ANTIMIKROBA PADA LUMUT HATI Dumortiera hirsuta Nees. DAN PERBANYAKANNYA DENGAN KULTUR JARINGAN
The resistance of infectious microbes is increasing. One alternative of reducing this resistance is by exploring plant-based natural anti-microbes on bryophytes. Based on the previous research, acetone and methanol-water extract of Dumortiera hirsuta in have shown inhibitory activities as antibacter...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/123700/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63814 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | The resistance of infectious microbes is increasing. One alternative of reducing
this resistance is by exploring plant-based natural anti-microbes on bryophytes. Based
on the previous research, acetone and methanol-water extract of Dumortiera hirsuta in
have shown inhibitory activities as antibacterial and antifungal. However its active
compound that have the highest inhibitory activities as antibacterial and antifungal have
not been elucidated. The problem of using the liverwort as a source of antimicrobial
compound was its low availability. Therefore, the objective of this research was to
identify the class of bioactive antimicrobial compound in liverwort and to produce the
antimicrobial compound by plant-tissue culture.
In this research, Dumortiera hirsuta that was taken from Cangar forest, Batu as
source of antimicrobial compound. Staphylococcus aureus ATCC 25923,Escherichia
coli ATCC 25922, and Candida albicans ATCC 10231 collections of laboratory of
Biology Department, Faculty of Science and Technology, Airlangga University were
used as test microbes. Dumortiera hirsuta was extracted using three solvents
chloroform, methanol, water. Antimicrobial activity of extracts were tested by using
disc diffusion and tube dillution methods. The best extract was fractioned by Vaccum
Liquid Chromatography and bioactive compounds are monitored by using Thin Layer
Chromatography (TLC). The most potential activity of the fractions was determined by
the same methods. The best fractions with highest inhibitory activities will be analyzed
by Preparative-TLC to detect the bioactive compound class. The explants of sporophyte
and gametophyte with various size (0,5 |
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