IDENTIFIKASI GOLONGAN SENYAWA ANTIMIKROBA PADA LUMUT HATI Dumortiera hirsuta Nees. DAN PERBANYAKANNYA DENGAN KULTUR JARINGAN

• Main Authors: , JUNAIRIAH, , Prof. Sukarti Moeljopawiro, M.AppSc., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123700/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63814
• Institution: Universitas Gadjah Mada

The resistance of infectious microbes is increasing. One alternative of reducing this resistance is by exploring plant-based natural anti-microbes on bryophytes. Based on the previous research, acetone and methanol-water extract of Dumortiera hirsuta in have shown inhibitory activities as antibacterial and antifungal. However its active compound that have the highest inhibitory activities as antibacterial and antifungal have not been elucidated. The problem of using the liverwort as a source of antimicrobial compound was its low availability. Therefore, the objective of this research was to identify the class of bioactive antimicrobial compound in liverwort and to produce the antimicrobial compound by plant-tissue culture. In this research, Dumortiera hirsuta that was taken from Cangar forest, Batu as source of antimicrobial compound. Staphylococcus aureus ATCC 25923,Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 collections of laboratory of Biology Department, Faculty of Science and Technology, Airlangga University were used as test microbes. Dumortiera hirsuta was extracted using three solvents chloroform, methanol, water. Antimicrobial activity of extracts were tested by using disc diffusion and tube dillution methods. The best extract was fractioned by Vaccum Liquid Chromatography and bioactive compounds are monitored by using Thin Layer Chromatography (TLC). The most potential activity of the fractions was determined by the same methods. The best fractions with highest inhibitory activities will be analyzed by Preparative-TLC to detect the bioactive compound class. The explants of sporophyte and gametophyte with various size (0,5