ISOLASI Rhizopus oryzae DARI BUAH BUSUK DAN UJI POTENSINYA DALAM PRODUKSI ASAM LAKTAT PADA MEDIA GLUKOSA DENGAN DAN TANPA PENAMBAHAN KALSIUM KARBONAT

• Main Authors: , TITIK KURNIAWATI, , Prof. Dr. Ir. Sardjono, MS.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/124591/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=64745
• Institution: Universitas Gadjah Mada

Lactic acid studies today are popular with its role as building blocks of Poly Lactic Acid (PLA) for manufacturing biodegradable plastic. Lactic acid bacteria (LAB) are well known as lactic acid producer, however, LAB are fastidious and require more complex nutrient for lactic acid fermentation. Studies of lactic acid production by filamentous fungi Rhizopus oryzae have been explored in the world, unfortunately, these studies are still limited in Indonesia, particularly studies in lactic acid production by indigenous strain R. oryzae. Four strains obtained from rotten avocado and guava were potential in producing lactic acid (AT1, JT1, AT2, and AT3). Rhizopus oryzae AT3 was used for lactic acid production using 100 g/l glucose medium with and without addition of 7,5 g/l calcium carbonate (CaCO3) at initial fermentation. Addition of CaCO3 increased lactic acid concentration of 59,30%, the concentrations were 11,61 g/l and 18,495 g/l in glucose medium and glucose medium with CaCO3 addition, respectively. Glucose+CaCO3 medium also showed higher productivity, reached continuously from 1 day (0,059 g/l/h) until 5 days fermentation (0,154 g/l/h), whereas highest productivity in glucose medium was reached at 1 day fermentation (0,124 g/l/h) and continued to decrease until 5 days fermentation (0,065 g/l/h)