PERAKITAN DAN PEMETAAN GEN KETAHANAN TERHADAP POWDERY MILDEW DENGAN PENANDA SEQUENCE CHARACTERIZED AMPLIFIED REGION PADA MELON (Cucumis melo L.) KULTIVAR TACAPA
Cultivation of melon ( Cucumis melo L. ) has been developed in Indonesia. Cultivated melon varieties have different advantages, but generally still vulnerable to disease. Powdery mildew is a disease that infects nearly all melon plants at the melon cultivation centers in Indonesia. The disease is la...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/126214/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66418 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Cultivation of melon ( Cucumis melo L. ) has been developed in Indonesia. Cultivated
melon varieties have different advantages, but generally still vulnerable to disease. Powdery
mildew is a disease that infects nearly all melon plants at the melon cultivation centers in
Indonesia. The disease is largely attributable to the fungus Podosphaera xanthii. Plant
breeding methods continue to be developed for producing superior melon seeds and resistant
to powdery mildew. In 2009, a new melon cultivar, called TACAPA has been produced and
it is resistant against powdery mildew. The cultivar has good fruit quality, however still show
segregation in second generation (F2). Assembly and mapping of resistance gene against
powdery mildew with SCAR marker on melon cultivars TACAPA are needed to produce
superior melon seeds of TACAPA. This study conducted crossing of TACAPA with PI
371795 and Action 343, so that the resulting two new generations are PI 371 795 x
TACAPA and TACAPA x Action 434. Melons from crosses were planted in a greenhouse as
much as 110 plants. Then the sample of the leaves were used for DNA extraction and
amplification with SCAR marker. DNA amplification products were analyzed by Chi-square
(X2) test. Chi-square test results were used to analysis linkage map with map markers. The
results showed that the pattern of inheritance of resistance genes against powdery mildew on
melons TACAPA controlled by a single dominant gene ( Pm-I). The position of SCAR
molecular marker linked to gene Pm-I resistance against powdery mildew on TACAPA x PI
371795 was 31,5 cM, while on TACAPA x Action 434 was 3, 7 cM. |
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