PERAKITAN DAN PEMETAAN GEN KETAHANAN TERHADAP POWDERY MILDEW DENGAN PENANDA SEQUENCE CHARACTERIZED AMPLIFIED REGION PADA MELON (Cucumis melo L.) KULTIVAR TACAPA

• Main Authors: , Ahdiat Agriansyah, , Dr. Budi Setiadi Daryono, M.Agr.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/126214/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66418
• Institution: Universitas Gadjah Mada

Cultivation of melon ( Cucumis melo L. ) has been developed in Indonesia. Cultivated melon varieties have different advantages, but generally still vulnerable to disease. Powdery mildew is a disease that infects nearly all melon plants at the melon cultivation centers in Indonesia. The disease is largely attributable to the fungus Podosphaera xanthii. Plant breeding methods continue to be developed for producing superior melon seeds and resistant to powdery mildew. In 2009, a new melon cultivar, called TACAPA has been produced and it is resistant against powdery mildew. The cultivar has good fruit quality, however still show segregation in second generation (F2). Assembly and mapping of resistance gene against powdery mildew with SCAR marker on melon cultivars TACAPA are needed to produce superior melon seeds of TACAPA. This study conducted crossing of TACAPA with PI 371795 and Action 343, so that the resulting two new generations are PI 371 795 x TACAPA and TACAPA x Action 434. Melons from crosses were planted in a greenhouse as much as 110 plants. Then the sample of the leaves were used for DNA extraction and amplification with SCAR marker. DNA amplification products were analyzed by Chi-square (X2) test. Chi-square test results were used to analysis linkage map with map markers. The results showed that the pattern of inheritance of resistance genes against powdery mildew on melons TACAPA controlled by a single dominant gene ( Pm-I). The position of SCAR molecular marker linked to gene Pm-I resistance against powdery mildew on TACAPA x PI 371795 was 31,5 cM, while on TACAPA x Action 434 was 3, 7 cM.