DEMINERALISASI CANGKANG RAJUNGAN PADA PROSES PEMBUATAN KITIN DENGAN Lactobacillus acidophilus

• Main Authors: , AHSANATUNSYAHIDAWATI, , Prihati Sih Nugraheni, S.Pi., M.P.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127274/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=67523
• Institution: Universitas Gadjah Mada

The purpose of this research was to study the decrease of mineral content on crab shell (Portunus sp.) using biological demineralization process. Biological demineralization process was done by incubating Lactobacillus acidophilus 10 ml on crab shells 30 gr in 100 ml medium that consisted of glucose 6 g/100 ml and yeast extract 0,05 g/100 ml at 30 ± 1ºC with 50 rpm agitation for the period of 0, 12, 24, 36, and 48 hours. The negative control was done with the same method with biological demineralization but without the addition of L. acidophilus. Chemical demineralization using 1.5 N HCl (1:12 w/v) was used as a positive control. The results showed that using L. acidophilus in 48 hours demineralization fermentation with ratio between crab shell and medium was (1:3, w/v) has not signification result reduction crab shell�s mineral content but the decreased of mineral content showed decreasing trend followed y= -0,1489x + 38,603 and r= 0,9983. The decreased of ash content occurred because pH medium decreased. The decreased of pH medium caused by the growth of lactid acid bacteria. Lactid acid bacteria showed that growing up to 12 hours fermentation later showed constant growth. Optimation in fermentation time and ratio between quantity of medium of fermentation and crab shell was needed to be studied further, in order to get the optimum lactid acid bacteria growth so could result optimum lactid acid that decrease pH and demineralization could be optimum.