INDUKSI PEMBUNGAAN TANAMAN ANGGREK Phalaenopsis amabilis (L) Blume DENGAN PERLAKUAN BENZILADENIN SECARA IN VITRO
Orchid are ornamental plants that grown cultivated for the flowers. One of the efforts to improve orchid breeding is by optimalizing cultivation techniques through flower forcing. The objective of this study is to accelerate flowering time through to understand the effect of Benzyladenine (BA) on fl...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/128661/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69024 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Orchid are ornamental plants that grown cultivated for the flowers. One
of the efforts to improve orchid breeding is by optimalizing cultivation techniques
through flower forcing. The objective of this study is to accelerate flowering time
through to understand the effect of Benzyladenine (BA) on flowering of
indonesian wild orchids Phalaenopsis amabilis (L.) Blume using in vitro culture.
The flowering induction was carried out by addition BA (0, 1, 3, 5, 7 and 9) ppm
growht regulator. The seeds were spreaded on New Phalaenopsis (NP) medium
containing 15% coconut milk for 8 weeks, then subcultured in liquid medium NP
+ BA treated with various concentrations of BA for 9 weeks with shaking. The
culture subsequently were transferred on to the 2 layer medium (solid: liquid =
4:1). The culture is maintained on 25�C with continue lighting. Observations were
made by counting the leaf and root length and weight, number of leaf and root
formed. The data were analyzed using ANOVA, α 0.05.
Flowering detection is done by RT - PCR (Reverse Transcriptase
Polymerase Chain Reaction) with specific gene of Phalaeonopsis Orchid
Hemeobox (POH1) and Phalaeonopsis amabilis Flowering Locus T (PaFT). To
determine whether PaFT gene had been activated in analyzed plant or not and for
comparison of the efficiency of the RT-PCR the expression of POH1 gene was
also performed for POH1 gene (juvenile gene). The accumulation of mRNA was
detected for both POH1 and PaFT genes in 28 weeks old plants. On the other
hand in nontreatment plants, only POH1 mRNA was detected. These data suggest
that BA treatment induced activation of PaFT gene in juvenile stage, but also
extend the activation the POH1 as juvenile gene. No flowering can be induced
yet, because of redundancy and antagonism between POH1 and PaFT genes
might be occurred, or any posttranscription or post translational events happened. |
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