INDUKSI PEMBUNGAAN TANAMAN ANGGREK Phalaenopsis amabilis (L) Blume DENGAN PERLAKUAN BENZILADENIN SECARA IN VITRO

• Main Authors: , BEKTI SULISTYA UTAMI, , Dr. Endang Semiarti, M.S., M.Sc
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/128661/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69024
• Institution: Universitas Gadjah Mada

Orchid are ornamental plants that grown cultivated for the flowers. One of the efforts to improve orchid breeding is by optimalizing cultivation techniques through flower forcing. The objective of this study is to accelerate flowering time through to understand the effect of Benzyladenine (BA) on flowering of indonesian wild orchids Phalaenopsis amabilis (L.) Blume using in vitro culture. The flowering induction was carried out by addition BA (0, 1, 3, 5, 7 and 9) ppm growht regulator. The seeds were spreaded on New Phalaenopsis (NP) medium containing 15% coconut milk for 8 weeks, then subcultured in liquid medium NP + BA treated with various concentrations of BA for 9 weeks with shaking. The culture subsequently were transferred on to the 2 layer medium (solid: liquid = 4:1). The culture is maintained on 25�C with continue lighting. Observations were made by counting the leaf and root length and weight, number of leaf and root formed. The data were analyzed using ANOVA, α 0.05. Flowering detection is done by RT - PCR (Reverse Transcriptase Polymerase Chain Reaction) with specific gene of Phalaeonopsis Orchid Hemeobox (POH1) and Phalaeonopsis amabilis Flowering Locus T (PaFT). To determine whether PaFT gene had been activated in analyzed plant or not and for comparison of the efficiency of the RT-PCR the expression of POH1 gene was also performed for POH1 gene (juvenile gene). The accumulation of mRNA was detected for both POH1 and PaFT genes in 28 weeks old plants. On the other hand in nontreatment plants, only POH1 mRNA was detected. These data suggest that BA treatment induced activation of PaFT gene in juvenile stage, but also extend the activation the POH1 as juvenile gene. No flowering can be induced yet, because of redundancy and antagonism between POH1 and PaFT genes might be occurred, or any posttranscription or post translational events happened.