PERTUMBUHAN DAN AKTIVITAS XILANASE ISOLAT KAPANG XILANOLITIK (STRAIN JKB) PADA SUBSTRAT SERASAH DAUN
Leaf litters were composed of cellulosic materials including celluloses, hemicelluloses and lignin. Hemicelluloses consisted of xylan, not easily degraded by microorganisms. Only several species of microorganisms, especially molds could use xylan as carbon sources for their growth. Some species of m...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/133076/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73628 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Leaf litters were composed of cellulosic materials including celluloses,
hemicelluloses and lignin. Hemicelluloses consisted of xylan, not easily degraded
by microorganisms. Only several species of microorganisms, especially molds
could use xylan as carbon sources for their growth. Some species of molds
released xylanase to hydrolyze xylan into xylose or xylo-olygosaccharides. This
enzyme was needed for paper industry, especially pulp bleaching proccesses. The
objective of this study were to obtain isolates capable to grow on leaf litter, to
observe xylanase activity produced by the isolates and to identify selected
isolates. The research was commenced by selection of molds isolates based on
capability to grow on solid medium containing xylan, and xylanolitic index using
congo red methode. Grown mold colonies showed a high xylanolitic index were
selected for further investigation. The ability of selected isolates to grow on liquid
medium was investigated through the growth experiment using liquid media
containing leaf litter powder or Birchwood xylan as carbon source. At interval of
time, fungal growth was measured based on their biomass (dry weight) |
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