KEMAMPUAN EKSTRAK TEMUMANGGA (Curcuma mangga) DALAM MENGHAMBAT PROSES OKSIDASI LOW DENSITY LIPOPROTEIN
The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis. Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation....
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| Main Authors: | , , , |
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| Format: | Article PeerReviewed |
| Language: | English |
| Published: |
FORUM PASCASARJANA
2010
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/135266/1/forum%20pascasarjana.pdf https://repository.ugm.ac.id/135266/ |
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| Institution: | Universitas Gadjah Mada |
| Language: | English |
| Summary: | The oxidation of low-density lipoprotein (LDL) is believed to be the initiating
factor for the development and progression of atherosclerosis. Curcuminoid, the
metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has
been shown to reduce the susceptibility of LDL to oxidation. In this study, we
examined the effect of curcuminoid extracted from temu mangga on copper ioninduced
lipid peroxidation of low density lipoprotein (LDL) in mice's macrophages
and Macaca nemestrina's monocytes. Analyses were done by measuring the
formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde
(MDA). LDL were harvested and isolated from 5 adult female Macaca fascicularis
fed aterogenic diet for 3 months. LDL oxidation by mice macrophage incubated for
4 hours were inhibited by curcuminoid at concentration of 8 ppm. There was
decreased 17% {P<O. 01) in the concentration of MDA compared to control without
curcuminoid (31.99"±0. vs 36.7T±0.9 nmollmg protein LDL, respectively).
lnhibiton of LDL oxidation in M. nemestrina macrophage were highest by
curcuminoid at 8 ppm for 4 hours and 6 hours incubation. There was 14.8% and
23% inhibiton (P<0.01) (23.768 ± 0.095A vs 27.1118±0.972 and 23.37"±0.12 vs
30. 8T±2.49 nmollmg LDL protein, respectively). These data suggest that curcuminoid of temu mangga were able to inhibit LDL oxidation in the cellular level,therefore offer protection against oxidation of LDL. |
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