A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
The isolation of plant resistance (R) genes provides opportunities to produce crop plant varieties with increased disease resistance. Previous techniques to clone plant R genes, including transposon tagging and map base cloning, are lime consuming and may require a knowledge of a location of R gene...
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| Format: | Article NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
1998
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| Online Access: | https://repository.ugm.ac.id/17636/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=397 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | The isolation of plant resistance (R) genes provides opportunities to produce crop plant varieties with increased disease resistance. Previous techniques to clone plant R genes, including transposon tagging and map base cloning, are lime consuming and may require a knowledge of a location of R gene in the genome. A new technique called Representational Difference Analysis (RDA) offers a straightforward method for cloning DNA fragments differences between tivo complex genomes. In this study, the technique was used to clone a DNA fragment found only in tomato plants CLycopersicon esculentura) which are resistant to Altemaria stem canker. This suggests that RDA could provide a rapid method of cloning disease resistance genes in plants.
Keywords: Plant resistance (R) gene - representational difference analysis (RDA) Altemaria stem canker tester amplicon � driver amplicon . |
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