A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen

The isolation of plant resistance (R) genes provides opportunities to produce crop plant varieties with increased disease resistance. Previous techniques to clone plant R genes, including transposon tagging and map base cloning, are lime consuming and may require a knowledge of a location of R gene...

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Main Author:	Perpustakaan UGM, i-lib
Format:	Article NonPeerReviewed
Published:	[Yogyakarta] : Universitas Gadjah Mada 1998
Subjects:	Jurnal i-lib UGM
Online Access:	https://repository.ugm.ac.id/17636/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=397
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Institution:	Universitas Gadjah Mada

id	id-ugm-repo.17636
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spelling	id-ugm-repo.176362014-06-18T00:37:35Z https://repository.ugm.ac.id/17636/ A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen Perpustakaan UGM, i-lib Jurnal i-lib UGM The isolation of plant resistance (R) genes provides opportunities to produce crop plant varieties with increased disease resistance. Previous techniques to clone plant R genes, including transposon tagging and map base cloning, are lime consuming and may require a knowledge of a location of R gene in the genome. A new technique called Representational Difference Analysis (RDA) offers a straightforward method for cloning DNA fragments differences between tivo complex genomes. In this study, the technique was used to clone a DNA fragment found only in tomato plants CLycopersicon esculentura) which are resistant to Altemaria stem canker. This suggests that RDA could provide a rapid method of cloning disease resistance genes in plants. Keywords: Plant resistance (R) gene - representational difference analysis (RDA) Altemaria stem canker tester amplicon â�� driver amplicon . [Yogyakarta] : Universitas Gadjah Mada 1998 Article NonPeerReviewed Perpustakaan UGM, i-lib (1998) A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=397
institution	Universitas Gadjah Mada
building	UGM Library
country	Indonesia
collection	Repository Civitas UGM
topic	Jurnal i-lib UGM
spellingShingle	Jurnal i-lib UGM Perpustakaan UGM, i-lib A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
description	The isolation of plant resistance (R) genes provides opportunities to produce crop plant varieties with increased disease resistance. Previous techniques to clone plant R genes, including transposon tagging and map base cloning, are lime consuming and may require a knowledge of a location of R gene in the genome. A new technique called Representational Difference Analysis (RDA) offers a straightforward method for cloning DNA fragments differences between tivo complex genomes. In this study, the technique was used to clone a DNA fragment found only in tomato plants CLycopersicon esculentura) which are resistant to Altemaria stem canker. This suggests that RDA could provide a rapid method of cloning disease resistance genes in plants. Keywords: Plant resistance (R) gene - representational difference analysis (RDA) Altemaria stem canker tester amplicon â�� driver amplicon .
format	Article NonPeerReviewed
author	Perpustakaan UGM, i-lib
author_facet	Perpustakaan UGM, i-lib
author_sort	Perpustakaan UGM, i-lib
title	A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
title_short	A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
title_full	A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
title_fullStr	A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
title_full_unstemmed	A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen
title_sort	representational difference analysis: a promising method to clone a dna fragment which is responsible on the resistance mechanism of a plant to a pathogen
publisher	[Yogyakarta] : Universitas Gadjah Mada
publishDate	1998
url	https://repository.ugm.ac.id/17636/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=397
_version_	1681217117756588032

A representational difference analysis: A promising method to clone a DNA fragment which is responsible on the resistance mechanism of a plant to a pathogen

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