Immunomodulatory Effect of Massoia Bark Extract and the Cytotoxicity Activity Against Fibroblast and Vero Cells in Vitro

• Main Authors: Permanasari, Prisci, Hertiani, Triana, Yuswanto, Agustinus
• Format: Article PeerReviewed
• Language: English
• Published: International Journal of Pharmaceutical and Clinical Research 2016
• Subjects: Clinical Pharmacy and Pharmacy Practice; Pharmacology and Pharmaceutical Sciences
• Online Access: https://repository.ugm.ac.id/273443/1/Immunomodulatory%20Effect%20of%20Massoia%20Bark%20Extract%20and%20the%20Cytotoxicity%20Activity%20Against%20Fibroblast%20and%20Vero%20Cells%20in%20Vitro-Triana%20Hertiani.pdf; https://repository.ugm.ac.id/273443/; http://ijpcr.com
• Institution: Universitas Gadjah Mada

Background: Bark of Massoia aromatica Becc (Lauraceae) has been widely used in Java, Bali and Sumatra as a fragrance ingredient, seasoning and traditional medicine. C-10 Massoia lactone is a major constituent of its essential oil which has been proven as the antibacterial principle and has the ability to increase the phagocytic index of mice macrophage. Objective: to find out the immunomodulatory effects of the Massoia aromatica bark extract against Candida albicans as well as to explore the potential toxicities against fibroblast and vero cells. Material and methods: The extract was obtained by maseration of the pulverized barks with ethanol 96%, followed by solvent evaporation. The Massoia lactone contain was analysed by TLC-Densitometry. The immunological assay was based on the effect on SD mice lymphocyte proliferation and macrophage phagocytosis activities against latex and Candida albicans. Samples concentration tested were in the range of 30 – 2.5 μg/mL and done in triplicate. Cytotoxicity assays were done on fibroblast and vero cells by using the MTT assay. ANOVA followed by Tukey-HSD was used for statistical analyses. Results: The ethanolic extract was separated into two layers, i.e. solid and oily phases. All samples exhibited no effect on lymphocyte cell proliferation which indicated by the stimulation index proliferation value of less than 2. However, all samples showed activation of the macrophage phagocytosis both on latex and Candida albicans in a concentration dependant manner. IC50s of oily and solid extracts on fibroblast were 46.13 and 53.03 μg/ml respectively, while on Vero cell were found at 43.59 and 153.05 μg/ml, respectively.