Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia

• Main Authors: Krisnawati, Ririn, Sardjono, Sardjono, Widada, Jaka, Suroto, Dian Anggraini, Cahyanto, Muhammad Nur
• Format: Article PeerReviewed
• Language: English
• Published: Journal of Pure and Applied Microbiology (JPAM) 2022
• Subjects: Microbiology
• Online Access: https://repository.ugm.ac.id/278767/1/Krisnawati_TP.pdf; https://repository.ugm.ac.id/278767/; https://microbiologyjournal.org/effect-of-glucose-on-endo-xylanase-and-%ce%b2-xylosidase-production-by-fungi-isolated-in-indonesia/; https://doi.org/10.22207/JPAM.16.1.12
• Institution: Universitas Gadjah Mada

Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endoxylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT 2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021-0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined. © The Author(s) 2022.