The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast
Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5-fluorouracil (5-FU) has already been use...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Research Center for Biotechnology
2022
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/283833/1/69505-270397-2-PB.pdf https://repository.ugm.ac.id/283833/ https://www.scopus.com/ |
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| Institution: | Universitas Gadjah Mada |
| Language: | English |
| Summary: | Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5-fluorouracil (5-FU) has already been used in keloid management. This study aimed to determine the efficacy of the combination of captopril and 5-FU in keloid fibroblast cultures. Keloid tissues were cultured up to passages 4-7. The study consisted of a control group, captopril in various concentrations (10-2, 10-3, 10-4 and 10-5 mol/L), 5-FU 1 mg/mL and a combination of captopril at various concentrations with 5-FU 1 mg/mL. After 144 hours of treatment, fibroblast proliferation and collagen deposition were measured. The study showed a significant decrease in the mean index of fibroblast proliferation and collagen deposition in the group receiving captopril in various concentrations (10-2, 10-3, 10-4 and 10-5 mol/L) and the 5-FU group against the control group (p<0.05). In the combined-dose group, captopril at a concentration of 10-2 mol/L and 5-FU showed a significant reduction in fibroblast proliferation and collagen deposition compared to the 5-FU group and the captopril at the same dose (p<0.05). In conclusion, the combination of captopril 10-2 mol/L and 5-FU 1 mg/mL is better at reducing fibroblast proliferation and collagen deposition in keloid fibroblast cultures than captopril or 5-FU as a single therapeutic agent. © 2022 THE AUTHOR(S). This article is distributed under a Creative Commons Attribution-ShareAlike 4.0 International license. |
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