The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast
Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5-fluorouracil (5-FU) has already been use...
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Universitas Gadjah Mada, Research Center for Biotechnology
2022
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id-ugm-repo.2838332023-11-23T01:09:45Z https://repository.ugm.ac.id/283833/ The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo Dermatology Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5-fluorouracil (5-FU) has already been used in keloid management. This study aimed to determine the efficacy of the combination of captopril and 5-FU in keloid fibroblast cultures. Keloid tissues were cultured up to passages 4-7. The study consisted of a control group, captopril in various concentrations (10-2, 10-3, 10-4 and 10-5 mol/L), 5-FU 1 mg/mL and a combination of captopril at various concentrations with 5-FU 1 mg/mL. After 144 hours of treatment, fibroblast proliferation and collagen deposition were measured. The study showed a significant decrease in the mean index of fibroblast proliferation and collagen deposition in the group receiving captopril in various concentrations (10-2, 10-3, 10-4 and 10-5 mol/L) and the 5-FU group against the control group (p<0.05). In the combined-dose group, captopril at a concentration of 10-2 mol/L and 5-FU showed a significant reduction in fibroblast proliferation and collagen deposition compared to the 5-FU group and the captopril at the same dose (p<0.05). In conclusion, the combination of captopril 10-2 mol/L and 5-FU 1 mg/mL is better at reducing fibroblast proliferation and collagen deposition in keloid fibroblast cultures than captopril or 5-FU as a single therapeutic agent. © 2022 THE AUTHOR(S). This article is distributed under a Creative Commons Attribution-ShareAlike 4.0 International license. Universitas Gadjah Mada, Research Center for Biotechnology 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283833/1/69505-270397-2-PB.pdf Amelia, Jesslyn and Siswati, Agnes Sri and Wirohadidjojo, Yohanes Widodo (2022) The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast. Indonesian Journal of Biotechnology, 27 (3). 126 -132. https://www.scopus.com/ 10.22146/ijbiotech.69505 |
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Dermatology Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
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Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5-fluorouracil (5-FU) has already been used in keloid management. This study aimed to determine the efficacy of the combination of captopril and 5-FU in keloid fibroblast cultures. Keloid tissues were cultured up to passages 4-7. The study consisted of a control group, captopril in various concentrations (10-2, 10-3, 10-4 and 10-5 mol/L), 5-FU 1 mg/mL and a combination of captopril at various concentrations with 5-FU 1 mg/mL. After 144 hours of treatment, fibroblast proliferation and collagen deposition were measured. The study showed a significant decrease in the mean index of fibroblast proliferation and collagen deposition in the group receiving captopril in various concentrations (10-2, 10-3, 10-4 and 10-5 mol/L) and the 5-FU group against the control group (p<0.05). In the combined-dose group, captopril at a concentration of 10-2 mol/L and 5-FU showed a significant reduction in fibroblast proliferation and collagen deposition compared to the 5-FU group and the captopril at the same dose (p<0.05). In conclusion, the combination of captopril 10-2 mol/L and 5-FU 1 mg/mL is better at reducing fibroblast proliferation and collagen deposition in keloid fibroblast cultures than captopril or 5-FU as a single therapeutic agent. © 2022 THE AUTHOR(S). This article is distributed under a Creative Commons Attribution-ShareAlike 4.0 International license. |
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Article PeerReviewed |
author |
Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo |
author_facet |
Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo |
author_sort |
Amelia, Jesslyn |
title |
The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
title_short |
The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
title_full |
The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
title_fullStr |
The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
title_full_unstemmed |
The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
title_sort |
efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
publisher |
Universitas Gadjah Mada, Research Center for Biotechnology |
publishDate |
2022 |
url |
https://repository.ugm.ac.id/283833/1/69505-270397-2-PB.pdf https://repository.ugm.ac.id/283833/ https://www.scopus.com/ |
_version_ |
1783956507679784960 |