Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase

Porous starches are essential modified starches that have potential application as natural absorbents and contain abundant micro sized pores. Increasing interest is directed to utilizing the properties of porous starches in various starchy foods, such as Canna edulis Kerr. To date, no report about e...

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Main Authors: Purwitasari, Lutfi, Wulanjati, Martha Purnami, Pranoto, Yudi, Witasari, Lucia Dhiantika
Format: Article PeerReviewed
Language:English
Published: Elsevier Ltd 2023
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Online Access:https://repository.ugm.ac.id/284133/1/1-s2.0-S2772753X2200140X-main.pdf
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Institution: Universitas Gadjah Mada
Language: English
id id-ugm-repo.284133
record_format dspace
institution Universitas Gadjah Mada
building UGM Library
continent Asia
country Indonesia
Indonesia
content_provider UGM Library
collection Repository Civitas UGM
language English
topic Food Sciences
spellingShingle Food Sciences
Purwitasari, Lutfi
Wulanjati, Martha Purnami
Pranoto, Yudi
Witasari, Lucia Dhiantika
Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
description Porous starches are essential modified starches that have potential application as natural absorbents and contain abundant micro sized pores. Increasing interest is directed to utilizing the properties of porous starches in various starchy foods, such as Canna edulis Kerr. To date, no report about edible canna porous starch is available. Therefore, this study aimed to characterize the porous starch from edible canna produced by enzymatic hydrolysis using thermostable α-amylase. A starch suspension (25, w/v) in phosphate buffer (0.1 M, pH 6.5) was added the thermostable α-amylase (100 U/g, 200 U/g and 300 U/g) and then incubated at 60 °C and 80 rpm for 8 h. The pellets were dried at 40 °C for 48 h. The SEM results displayed that the increase in enzyme concentration caused the formation of larger pores on the starch granule surface due to amylose degradation. FTIR and XRD analysis showed that enzymatic hydrolysis did not affect the crystalline structure of the edible canna porous starch. Furthermore, the edible canna porous starch exhibited high solubility and oil and water absorption capacity. Thus, the porous starch from C. edulis Kerr. could be used as a probiotic, flavoring, or drug delivery agent by microencapsulation.
format Article
PeerReviewed
author Purwitasari, Lutfi
Wulanjati, Martha Purnami
Pranoto, Yudi
Witasari, Lucia Dhiantika
author_facet Purwitasari, Lutfi
Wulanjati, Martha Purnami
Pranoto, Yudi
Witasari, Lucia Dhiantika
author_sort Purwitasari, Lutfi
title Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
title_short Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
title_full Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
title_fullStr Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
title_full_unstemmed Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
title_sort characterization of porous starch from edible canna (canna edulis kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase
publisher Elsevier Ltd
publishDate 2023
url https://repository.ugm.ac.id/284133/1/1-s2.0-S2772753X2200140X-main.pdf
https://repository.ugm.ac.id/284133/
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spelling id-ugm-repo.2841332023-11-28T08:24:55Z https://repository.ugm.ac.id/284133/ Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase Purwitasari, Lutfi Wulanjati, Martha Purnami Pranoto, Yudi Witasari, Lucia Dhiantika Food Sciences Porous starches are essential modified starches that have potential application as natural absorbents and contain abundant micro sized pores. Increasing interest is directed to utilizing the properties of porous starches in various starchy foods, such as Canna edulis Kerr. To date, no report about edible canna porous starch is available. Therefore, this study aimed to characterize the porous starch from edible canna produced by enzymatic hydrolysis using thermostable α-amylase. A starch suspension (25, w/v) in phosphate buffer (0.1 M, pH 6.5) was added the thermostable α-amylase (100 U/g, 200 U/g and 300 U/g) and then incubated at 60 °C and 80 rpm for 8 h. The pellets were dried at 40 °C for 48 h. The SEM results displayed that the increase in enzyme concentration caused the formation of larger pores on the starch granule surface due to amylose degradation. FTIR and XRD analysis showed that enzymatic hydrolysis did not affect the crystalline structure of the edible canna porous starch. Furthermore, the edible canna porous starch exhibited high solubility and oil and water absorption capacity. Thus, the porous starch from C. edulis Kerr. could be used as a probiotic, flavoring, or drug delivery agent by microencapsulation. Elsevier Ltd 2023 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/284133/1/1-s2.0-S2772753X2200140X-main.pdf Purwitasari, Lutfi and Wulanjati, Martha Purnami and Pranoto, Yudi and Witasari, Lucia Dhiantika (2023) Characterization of porous starch from edible canna (Canna edulis Kerr.) produced by enzymatic hydrolysis using thermostable alpha-amylase. Food Chemistry Advances, 2. ISSN 2772753X https://pdf.sciencedirectassets.com/780605/1-s2.0-S2772753X22X00038/1-s2.0-S2772753X2200140X/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEF8aCXVzLWVhc3QtMSJIMEYCIQCU6QBLAXfg3tucA%2BIywfRNXfktgiV%2BItrGbcyTSqO9jgIhAMkp0ZQnmyrxChfkKReZjjQ%2BP7Usr86dBcVUGrj6mYqLKrsFCLj%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEQBRoMMDU5MDAzNTQ2ODY1IgwK1uDnN3wXS5yYRQ8qjwU0iPmSnkRHq2Ji8xvU2a6BK5571OOqSLuCOMa7vqmgAQF6oHlSsvGi8CkE1VWcnQtxtSRqhRr9Hd%2FdyUzrvKKVgXh3V%2F1HzGu6kasYe%2FJdxaPa7NiDfv%2BUpnIf6qjtWPWa04SDEqZtDW%2BdYYwmfh7AvXsdxhRYRssoLnNJsO7889vzGZWvQYCeLX2MbfRUvBkFVN2BiOG%2Bb94jw6hlqAXhB7s6qOMwWmtjH0KNfsd28WbiEqhShptQTJvj%2FyO3fRXVOEZjL3KooLHRXcrNKSp0VcHPzruUWV1QdHclygcts30EP1Cb8yhTUeJymmU7Rqxa%2BpAdiMmDGUAYX0MzSmHa%2FqFEL1UA2UWzHlsyYqo7zy5a%2BiTiaqhdKyc8xjcHEL4Q5L57K1C%2BdtVbh2qyBE8%2FeThLJJsZalR9V1gD9HxOuLTRr1lX1wZOjIK8pn0ANsPfNwfa%2BQvX0NetknANYJmo2anFqcCMM6Jq%2FQa084JsfqwXrCr2J3tKAk4hdgtR%2FZwfnkMLm%2FxaMSzq2UlAOpuxjFRrNZPH5yRuYHPJYkBRfQYzd%2FzUhB4j%2F1KAUaPhQHbS7tUs%2BlvmUiJ%2BeO4tQCva0Lz4sHCnHwvr87Yf51ZRaHdGAdtk2yKeu5pcD8YcFUWxq%2BdfZeKxfWBvfxDWcZdrFVty3Re3YKZh3osKwgor3pgoxFhejzeEFq%2Bet%2BaOu8c4Iog8Dem1x0UvNrwBl6mjchXijHE9GXC5%2BmaAmpHydAG1fiVblWPZGYbixibpGu0EwxDcUHNmh%2F%2Fd0BRcpwf06qbNnCbZ0oHu2p0LBBSAh%2FGxTEmio%2F6mV61rAN%2BTvGuUmCw4SlnhN46mJjFHCBHoeGIoPJW0mbN75MzgWXYnMPielqsGOrABteF2%2BmLtRzID2f%2F5NtLZRTCgSydKHuRRylt5Q1E43BfBD%2BQ2Kp%2FQRyKSR%2F3ymZouMdfIkmt0YA85EEGtVqCbEpGOVPk4W1WnKL3w4LiCAMwHKEmfG%2FswVxzK8lVgXFmZcYt%2FCOgF6hS4pheNZ8l4IO%2F%2BFnjm%2BigKKePa3hmWcMVevsRBLsE8FdIB36eiL%2Fa87ILM7nOgNKPp8yxczFutYUlnVuvMlPdaVqS%2B4as32%2Fs%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20231128T080123Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY55TFHSGH%2F20231128%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=18759f5d218195610ea2af371e4ac243499a0b3976488ec4228ab72d13acf718&hash=4488c6066e77aaa723e6a3003e8590f9387ccf14f688f7760346fe9eef7b4d6f&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S2772753X2200140X&tid=spdf-ebf921ad-bcbd-4efe-a75c-26549958291b&sid=0be2e50f718bc644a31846605fb6be34f582gxrqb&type=client&tsoh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&ua=0f045e53055053515350&rr=82d115262970a309&cc=id 10.1016/j.focha.2022.100152