PEMANTAUAN LONGITUDINAL TITER ANTIBODI TERHADAP PROTEIN PERMUKAAN MEROZOIT Plasmodium falciparum PADA ANAK SEKOLAH DASAR DI KABUPATEN PURWOREJO, PROPINSI JAWA TENGAH

• Main Authors: , ANIS NUR WIDAYATI, , Prof. dr. Supargiyono, DTM&H., SU., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/88542/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50839
• Institution: Universitas Gadjah Mada

Background: Measurement of antibody can be used to estimate malaria transmission because the levels of antibodies depend on exposure to Plasmodiuminfected mosquitoes. Serological markers that have been widely used to estimate the malaria transmission in endemic areas is the MSP-1, MSP-2 and AMA-1. To find out how long the antibodies anti-MSP-1 and AMA-1 persist in the serum of primary school children in low endemic areas, research was done on elementary school children in Purworejo regency, Central Java Province. Objective: To determine the persistence of antibodies anti Pf.MSP-1 and Pf.AMA-1 in the serum of elementary school children living in low endemic areas, to know the seroprevalence, monthly fluctuations of antibody titer anti MSP-1 and AMA-1 titer of elementary school children in LCI area in Purworejo. Method: This research was observational analytic using cohort study design. Antibody titers were observed in serum samples monthly for 7 months observation periode (December 2008 � Juni 2009). Elisa were performed for all samples 483 children 4 and 5 grade elementary school collected in December 2008, and the sample to be tested monthly for Elisa was 160 samples. Result: Seroprevalence of IgG antibodies anti Pf.MSP-1 in 3 districts was 13,872%, and Pf.AMA-1 was 7,453 %. The persistence of antibodies anti Pf.AMA-1 and MSP-1 in the serum were dominated by the persistence of antibodies for 1 month, and can last longer than 1 - 7 months. Antibody anti Pf.MSP-1 and Pf.AMA-1 titers began to increase during the first month, decreased in the second month after infection and remained up to 6 months of observation time. Conclusion: Serologic marker can be used to determined the malaria seroprevalence, the persistence of anti Pf.MSP-1 and Pf.AMA-1, and the fluctuations of antibody anti MSP-1 and AMA-1 titers in serum of children in low endemic areas in Purworejo Region