RESEPTIVIT AS ENDOMETRIUM PENDERITA ENDOMETRIOSIS Kajian Biologi Molekuler: Ekspresl Mucin-1, Leukemia Inhibitory Factor, Cyclooxygenase-2 dan Polimorfisme Gena Mucin-1, Cyclooxygenase-2

• Main Author: , Uki Retno Budihastuti
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Program Pascasarjana, Universitas Gadjah Mada 2012
• Online Access: https://repository.ugm.ac.id/95305/; http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=3123
• Institution: Universitas Gadjah Mada

<p>Endometriosis is a disease affected by estrogen hormones, which also indicated by the existence of endometrium tissues or similarly an endometrium implanted tissue outside the cavum uterine and resulting to inflammation. In women with endometriosis, the endometrial receptivity is declining due to severalfactors, hence the conditio,!in the successfor embrio Implmltation becomes distracted. Embryo Implantationrequires the closely harmonized processes of apposition, attachment, and adhesion of the conceptus to the maternal endometrial epithelium. MUC-I, COX-2 and LlF are produced by the endometrium and are absolutely required for implantation.<br /> Case-control freqwently mentiOlfed as retrospective study with dependellt variable of endometriosis, endometriosis stadium, and endometrial receptivity. The dependent variables were assessed when diagllosing endometriosis based olliaparoscopic alld clinical examinations.The objective of this case-control study is to investigate endometrium receptivity disorders 0n endometriosispatients relating defects endometrium receptivity decline at secretory phase (the day 19th-24th).The research samples was takenfrolll endometr'iosispatients whose IIlIder laparoscopic surgery and the nor'mal patients whe Casensterilized, in which of each sample was 35 endometriosis patients and 32 nOli endometriosis patients.<br /> Immunohistochemistry methode was conductedfor examining the expression of MUC-I, COX-2 and LlF takes endometl'ialbiopsy at secretory phase (the day 19th-24th) by Immunohistochemistry. Examining MUC-I polymorphism by Amplification Refractory Mlltation System (ARMS) and Gene COX-2 Polymorphism by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP).<br /> The research's result show the increas of MUC-l expression in endometriosispatienls wilh OR=14,84: Cl 95%=1,25-175,51:p=O,03. The higher MUC-l expressionon endometriosispatients causes the difference in endometrialreceptivity.A ralher higher MUC-l expression on endometriosis patients affects Ihe emblYoadhesion during implan/ation to Ihe endometrium wall. The assesment result of COX-2 expression between Ihe endomelriosis group and conlrolled group have significant differel.lceswith OR=8,50,' CI 95%=2,06-35,08:p=O,OI.This result shows that the COX-2 expression 011 endomelriosis is higher than, controlled group. The endometriosis LIF expressionwas lower Ihan controlledgroup significantly wllh OR=J2,35: Cf 95%=3,84-39,76:p=O,OI.LlF role as biomarker In Implantation shown on the proliferation and secretion phases, in which the LIF expression is escalaling al the window of implan/a/ion because of the progesterone. The LIF expression change resulting in the disturbance of embryo invasion process.<br /> There is not any significanl difference between genotype AA, GA and GG towards the MUC-l expression between two groups. Alele A distribution and alele G of MUC-l were not different significantly. There was relationship belween genotype GC and GG with COX-2 expression wich was different significantly with OR=J,94: CI 95%=0,57-6,58,' p=O,08. It shown that the endometriosis had higher GC than controlled group. At the combination lest of MGC genolype wilh OR=6,43: CI95%=J,09-37,62,' p=O,OI. It also goes to Ihe GAGG group Illal has OR =3, 0: .91. 95~=O,84-JO,67: p=O,07 and refference AAGG as the normal genotype combinalion. II was concluded Ihal MUC-l expression 011endometriosis is lIigller Ihan control. COX-2 expression on endomelrlosis is higher Ihan control. LfF expression on endometriosis is lower Illan conlrol. Polymorphism frequency distribution of MUC-l and COX-2 are nol differenl but Ihere are differentiations in frequency distribution of genotype combination, which AAGC is higher on endometriosisthan control andsignifican</p>