Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
Lactobacillus spp. appearance in the oral cavity was suggested as a relevant target in order to coordinate a microbial balance and to counteract pathogenic bacteria, meanwhile prevent the onset of periodontal disease. This study aims to evaluate potential of probiotic bacteria as bacteriotherapy a...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
2017
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Subjects: | |
Online Access: | http://eprints.unisza.edu.my/5814/1/FH02-FSK-18-13128.pdf http://eprints.unisza.edu.my/5814/ |
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Institution: | Universiti Sultan Zainal Abidin |
Language: | English |
Summary: | Lactobacillus spp. appearance in the oral cavity was suggested as a relevant target in order to coordinate a
microbial balance and to counteract pathogenic bacteria, meanwhile prevent the onset of periodontal disease.
This study aims to evaluate potential of probiotic bacteria as bacteriotherapy against the Gram negative
periodontal pathogens, Aggregatibacter actinomycetemcomitan and Porphyromonas gingivalis. Eleven species
of probiotic bacteria were screened for inhibition activity against A. actinomycetemcomitans and P. gingivalis
using an overlay agar method. Probiotic bacteria with inhibition activity were subjected to indirect co-culture
assay. Then, the bactericidal activity against A. actinomycetemcomitans was evaluated for morphological
changes using Scanning Electron Microscope (SEM). In addition to this, biofilm deformation of A.
actinomycetemcomitans was also evaluated by co-culturing the probiotic bacteria with A.
actinomycetemcomitans and the biofilm formation was compared with A. actinomycetemcomitans without
probiotic bacteria (monoculture). Our finding showed out of eleven probiotic strains subjected for indirect coculture, three strains showed bactericidal effect against A. actinomycetemcomitans. They were Lactobacillus
plantarum NBRC 15891, Lactobacillus johnsonii NBRC 13952 and Leuconostoc fructosum NBRC 3516 with
growth reduction value 4.18, 4.77 and 3.6 log CFU/ml respectively. Whereas the bactericidal effect against P.
gingivalis was showed by two strains; L. plantarum NBRC 15891 and L. johnsonii NBRC 13952. The growth
reduction values were 9.5 and 9.1 log CFU/ml respectively. Morphological observation of
A.actinomycetemcomitans and P. gingivalis cells treated with indirect co-culture L. plantarum NBRC 15891 and
L. johnsonii NBRC 13952 showed collapsed and smaller in size compared to control. The biofilm deformation
of A. actinomycetemcomitans showed up to 3-fold reduction in the co-culture with probiotic strains compared to
mono-culture. In conclusion, this probiotic strains owns great potential as a candidate for bacteriotherapy in
controlling A.actinomycetemcomitans growth in-vitro. |
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