Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen

Lactobacillus spp. appearance in the oral cavity was suggested as a relevant target in order to coordinate a microbial balance and to counteract pathogenic bacteria, meanwhile prevent the onset of periodontal disease. This study aims to evaluate potential of probiotic bacteria as bacteriotherapy a...

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Main Authors: Norzawani, Jaffar, Toshinari, Maeda, Kouhei, Mizuno
Format: Article
Language:English
Published: 2017
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Online Access:http://eprints.unisza.edu.my/5814/1/FH02-FSK-18-13128.pdf
http://eprints.unisza.edu.my/5814/
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Institution: Universiti Sultan Zainal Abidin
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spelling my-unisza-ir.58142022-02-27T03:01:05Z http://eprints.unisza.edu.my/5814/ Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen Norzawani, Jaffar Toshinari, Maeda Kouhei, Mizuno QR Microbiology R Medicine (General) Lactobacillus spp. appearance in the oral cavity was suggested as a relevant target in order to coordinate a microbial balance and to counteract pathogenic bacteria, meanwhile prevent the onset of periodontal disease. This study aims to evaluate potential of probiotic bacteria as bacteriotherapy against the Gram negative periodontal pathogens, Aggregatibacter actinomycetemcomitan and Porphyromonas gingivalis. Eleven species of probiotic bacteria were screened for inhibition activity against A. actinomycetemcomitans and P. gingivalis using an overlay agar method. Probiotic bacteria with inhibition activity were subjected to indirect co-culture assay. Then, the bactericidal activity against A. actinomycetemcomitans was evaluated for morphological changes using Scanning Electron Microscope (SEM). In addition to this, biofilm deformation of A. actinomycetemcomitans was also evaluated by co-culturing the probiotic bacteria with A. actinomycetemcomitans and the biofilm formation was compared with A. actinomycetemcomitans without probiotic bacteria (monoculture). Our finding showed out of eleven probiotic strains subjected for indirect coculture, three strains showed bactericidal effect against A. actinomycetemcomitans. They were Lactobacillus plantarum NBRC 15891, Lactobacillus johnsonii NBRC 13952 and Leuconostoc fructosum NBRC 3516 with growth reduction value 4.18, 4.77 and 3.6 log CFU/ml respectively. Whereas the bactericidal effect against P. gingivalis was showed by two strains; L. plantarum NBRC 15891 and L. johnsonii NBRC 13952. The growth reduction values were 9.5 and 9.1 log CFU/ml respectively. Morphological observation of A.actinomycetemcomitans and P. gingivalis cells treated with indirect co-culture L. plantarum NBRC 15891 and L. johnsonii NBRC 13952 showed collapsed and smaller in size compared to control. The biofilm deformation of A. actinomycetemcomitans showed up to 3-fold reduction in the co-culture with probiotic strains compared to mono-culture. In conclusion, this probiotic strains owns great potential as a candidate for bacteriotherapy in controlling A.actinomycetemcomitans growth in-vitro. 2017-12 Article PeerReviewed text en http://eprints.unisza.edu.my/5814/1/FH02-FSK-18-13128.pdf Norzawani, Jaffar and Toshinari, Maeda and Kouhei, Mizuno (2017) Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen. Research Journal of Pharmacy and Technology, 10 (12). p. 28. ISSN 0974-3618
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic QR Microbiology
R Medicine (General)
spellingShingle QR Microbiology
R Medicine (General)
Norzawani, Jaffar
Toshinari, Maeda
Kouhei, Mizuno
Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
description Lactobacillus spp. appearance in the oral cavity was suggested as a relevant target in order to coordinate a microbial balance and to counteract pathogenic bacteria, meanwhile prevent the onset of periodontal disease. This study aims to evaluate potential of probiotic bacteria as bacteriotherapy against the Gram negative periodontal pathogens, Aggregatibacter actinomycetemcomitan and Porphyromonas gingivalis. Eleven species of probiotic bacteria were screened for inhibition activity against A. actinomycetemcomitans and P. gingivalis using an overlay agar method. Probiotic bacteria with inhibition activity were subjected to indirect co-culture assay. Then, the bactericidal activity against A. actinomycetemcomitans was evaluated for morphological changes using Scanning Electron Microscope (SEM). In addition to this, biofilm deformation of A. actinomycetemcomitans was also evaluated by co-culturing the probiotic bacteria with A. actinomycetemcomitans and the biofilm formation was compared with A. actinomycetemcomitans without probiotic bacteria (monoculture). Our finding showed out of eleven probiotic strains subjected for indirect coculture, three strains showed bactericidal effect against A. actinomycetemcomitans. They were Lactobacillus plantarum NBRC 15891, Lactobacillus johnsonii NBRC 13952 and Leuconostoc fructosum NBRC 3516 with growth reduction value 4.18, 4.77 and 3.6 log CFU/ml respectively. Whereas the bactericidal effect against P. gingivalis was showed by two strains; L. plantarum NBRC 15891 and L. johnsonii NBRC 13952. The growth reduction values were 9.5 and 9.1 log CFU/ml respectively. Morphological observation of A.actinomycetemcomitans and P. gingivalis cells treated with indirect co-culture L. plantarum NBRC 15891 and L. johnsonii NBRC 13952 showed collapsed and smaller in size compared to control. The biofilm deformation of A. actinomycetemcomitans showed up to 3-fold reduction in the co-culture with probiotic strains compared to mono-culture. In conclusion, this probiotic strains owns great potential as a candidate for bacteriotherapy in controlling A.actinomycetemcomitans growth in-vitro.
format Article
author Norzawani, Jaffar
Toshinari, Maeda
Kouhei, Mizuno
author_facet Norzawani, Jaffar
Toshinari, Maeda
Kouhei, Mizuno
author_sort Norzawani, Jaffar
title Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
title_short Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
title_full Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
title_fullStr Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
title_full_unstemmed Probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
title_sort probiotic as bacteriotherapy candidate against gram-negative periodontopathogen
publishDate 2017
url http://eprints.unisza.edu.my/5814/1/FH02-FSK-18-13128.pdf
http://eprints.unisza.edu.my/5814/
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