Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats

Background: The combination of S. salivarius K12 and M. acuminata are being used as synbiotic, but its safety evaulation is required. Objective: This study aimed to determine the LD50 of synbiotic containing probiotic Streptococcus salivarius K12 and prebiotic Musa acuminata peel extract. Materials...

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Main Authors: Mohd Fuad, Aalina Sakiina, Arzmi, Mohd Hafiz, Bakar, Muhammad Ekmal, Taib, Izatus Shima, Khuda, Fazle, Nasruddin, Nurrul Shaqinah
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Language:English
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English
Published: Elsevier B.V. on behalf of Institute of Transdisciplinary Health Sciences and Technology and World Ayurveda Foundation 2024
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https://doi.org/10.1016/j.jaim.2024.101037
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spelling my.iium.irep.1177692025-01-13T00:21:11Z http://irep.iium.edu.my/117769/ Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats Mohd Fuad, Aalina Sakiina Arzmi, Mohd Hafiz Bakar, Muhammad Ekmal Taib, Izatus Shima Khuda, Fazle Nasruddin, Nurrul Shaqinah QR Microbiology RK Dentistry Background: The combination of S. salivarius K12 and M. acuminata are being used as synbiotic, but its safety evaulation is required. Objective: This study aimed to determine the LD50 of synbiotic containing probiotic Streptococcus salivarius K12 and prebiotic Musa acuminata peel extract. Materials and Methods: The determination of LD50 is done according to the Acute Oral Toxicity test No. 425 (AOT425). For limit test, five female Sprague Dawley rats were given a limit dose of 2000 mg/kg of the synbiotic mixture once orally, and observed for 12 days. For subacute toxicity test, twenty female Sprague Dawley rats were randomised into 4 groups (n =5). Control group received saline, others received synbiotic mixture at doses 175 mg/kg, 550 mg/kg, and 2000 mg/kg, respectively, and observed for 14 days. Animals were euthanised on day-15, blood was collected, and subjected to haematological and biochemical analyses. Kidney and liver were preserved for histopathological examination. Result: No significant changes on the average body weight of the animals throughout the study. Haematological parameters and biochemical analysis do not depict any changes related to acute toxicity. Histopathology analysis depicted mild changes on kidney and liver. Conclusion: Based on the data, the LD50 of the synbiotic formulation is higher than 2000 mg/kb, with no sign of acute toxicity observed on all parameters. Elsevier B.V. on behalf of Institute of Transdisciplinary Health Sciences and Technology and World Ayurveda Foundation 2024-12-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/117769/7/117769_Acute%20oral%20toxicity%20evaluation%20of%20synbiotic.pdf application/pdf en http://irep.iium.edu.my/117769/13/117769_Acute%20oral%20toxicity%20evaluation%20of%20synbiotic_Scopus.pdf application/pdf en http://irep.iium.edu.my/117769/14/117769_Acute%20oral%20toxicity%20evaluation%20of%20synbiotic_WoS.pdf Mohd Fuad, Aalina Sakiina and Arzmi, Mohd Hafiz and Bakar, Muhammad Ekmal and Taib, Izatus Shima and Khuda, Fazle and Nasruddin, Nurrul Shaqinah (2024) Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats. Journal of Ayurveda and Integrative Medicine, 16 (1). pp. 1-6. ISSN 09759476 https://doi.org/10.1016/j.jaim.2024.101037 10.1016/j.jaim.2024.101037
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic QR Microbiology
RK Dentistry
spellingShingle QR Microbiology
RK Dentistry
Mohd Fuad, Aalina Sakiina
Arzmi, Mohd Hafiz
Bakar, Muhammad Ekmal
Taib, Izatus Shima
Khuda, Fazle
Nasruddin, Nurrul Shaqinah
Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats
description Background: The combination of S. salivarius K12 and M. acuminata are being used as synbiotic, but its safety evaulation is required. Objective: This study aimed to determine the LD50 of synbiotic containing probiotic Streptococcus salivarius K12 and prebiotic Musa acuminata peel extract. Materials and Methods: The determination of LD50 is done according to the Acute Oral Toxicity test No. 425 (AOT425). For limit test, five female Sprague Dawley rats were given a limit dose of 2000 mg/kg of the synbiotic mixture once orally, and observed for 12 days. For subacute toxicity test, twenty female Sprague Dawley rats were randomised into 4 groups (n =5). Control group received saline, others received synbiotic mixture at doses 175 mg/kg, 550 mg/kg, and 2000 mg/kg, respectively, and observed for 14 days. Animals were euthanised on day-15, blood was collected, and subjected to haematological and biochemical analyses. Kidney and liver were preserved for histopathological examination. Result: No significant changes on the average body weight of the animals throughout the study. Haematological parameters and biochemical analysis do not depict any changes related to acute toxicity. Histopathology analysis depicted mild changes on kidney and liver. Conclusion: Based on the data, the LD50 of the synbiotic formulation is higher than 2000 mg/kb, with no sign of acute toxicity observed on all parameters.
format Article
author Mohd Fuad, Aalina Sakiina
Arzmi, Mohd Hafiz
Bakar, Muhammad Ekmal
Taib, Izatus Shima
Khuda, Fazle
Nasruddin, Nurrul Shaqinah
author_facet Mohd Fuad, Aalina Sakiina
Arzmi, Mohd Hafiz
Bakar, Muhammad Ekmal
Taib, Izatus Shima
Khuda, Fazle
Nasruddin, Nurrul Shaqinah
author_sort Mohd Fuad, Aalina Sakiina
title Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats
title_short Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats
title_full Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats
title_fullStr Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats
title_full_unstemmed Acute oral toxicity evaluation of synbiotic mixture containing Streptococcus salivarius K12 and Musa acuminata aqueous peel extract in Sprague-Dawley rats
title_sort acute oral toxicity evaluation of synbiotic mixture containing streptococcus salivarius k12 and musa acuminata aqueous peel extract in sprague-dawley rats
publisher Elsevier B.V. on behalf of Institute of Transdisciplinary Health Sciences and Technology and World Ayurveda Foundation
publishDate 2024
url http://irep.iium.edu.my/117769/7/117769_Acute%20oral%20toxicity%20evaluation%20of%20synbiotic.pdf
http://irep.iium.edu.my/117769/13/117769_Acute%20oral%20toxicity%20evaluation%20of%20synbiotic_Scopus.pdf
http://irep.iium.edu.my/117769/14/117769_Acute%20oral%20toxicity%20evaluation%20of%20synbiotic_WoS.pdf
http://irep.iium.edu.my/117769/
https://doi.org/10.1016/j.jaim.2024.101037
_version_ 1821105166097580032