Development of process conditions to optimize the extraction of bio-disinfectant from Neem Leaf (Azadirachta Indica)

• Main Authors: Jamal, Parveen, Al-Mamun, Abdullah, Wan Chik, Wan Dalila, Zainuddin, Zaki, Mirghani, Mohamed Elwathig Saeed
• Format: Article
• Language: English
• Published: American-Eurasian Network for Scientific Information 2012
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/25020/1/development_of_process.pdf; http://irep.iium.edu.my/25020/; http://www.aensiweb.com/anas.html; http://www.aensiweb.com/anas/2012/682-686.pdf
• Institution: Universiti Islam Antarabangsa Malaysia

Central composite design (CCD) under Response surface methodology (RSM) was used in the present study to optimize the extraction parameters for assessing, maximum yield of bio-disinfectant content from Neem leaves extract (NLE). The range of the independent variables, namely solid-solvents ratio (0.05-0.1 w/v), extraction time (1-5 hrs), agitation speed (50-250 rpm) and extraction temperature (35-60 °C) were identified by a first set of four factors and five levels experiments. The optimum conditions for extraction of bio-disinfectant content from NLE were found to be at 75% ethanol with solid solvent ratio 0.067, extraction time 3 hrs, agitation speed 150 rpm and extraction temperature 60 °C. Under these optimized conditions, the maximum activity for bio-disinfectant yield was obtained, which showed 5.57 cm2 inhibition zones for anti-microbial assay. The experimental result was in close agreement with predicted values, thus indicating the suitability of the models developed and the success of RSM in optimizing the extraction conditions.