Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity

• Main Authors: Khatib, Alfi, Hoek, Arie C., Jinap, Selamat, Sarker, Md. Zaidul Islam, Jaswir, Irwandi, Verpoorte, Robert
• Format: Article
• Language: English
• Published: Taylor and Francis 2010
• Subjects: QD Chemistry
• Online Access: http://irep.iium.edu.my/33317/1/khatib_et_al%2C_2010._JLCRT.pdf; http://irep.iium.edu.my/33317/; http://www.tandfonline.com/toc/ljlc20/current#.UqV9euKuqKI
• Institution: Universiti Islam Antarabangsa Malaysia

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar=semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform=methanol (9=1) as the first mobile phase and ethyl acetate=chloroform=methanol (28=28=44) as the second mobile phase.